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部分牡丹品种遗传多样性的AFLP分析
引用本文:侯小改,尹伟伦,李嘉珏,王华芳.部分牡丹品种遗传多样性的AFLP分析[J].中国农业科学,2006,39(8):1709-1715.
作者姓名:侯小改  尹伟伦  李嘉珏  王华芳
作者单位:河南科技大学 北京林业大学
基金项目:河南省自然科学基金;河南省科技攻关项目
摘    要:【目的】研究牡丹遗传多样性为合理利用牡丹种质资源、培育观赏价值高的牡丹新品种提供依据。【方法】利用荧光标记AFLP技术,采用8对M+3和P+3引物组合对30份牡丹栽培品种进行了总基因组DNA水平上的多态性检测。【结果】检测结果共获得1 123条可统计的条带,其中965条呈多态性,多态性带百分率达86%。揭示了牡丹栽培种质丰富的遗传多样性。8组引物在30个品种中检测到数目不等的品种特异带型,这些品种的特异带型对供试牡丹品种具有一定的鉴别价值。【结论】8对引物能将30个牡丹品种完全区分开。聚类分析结果表明多数来源地相同的牡丹种质表现出较为密切的亲缘关系。

关 键 词:牡丹  遗传多样性  AFLP分析
收稿时间:2006-02-14
修稿时间:2006-02-142006-05-22

AFLP Analysis of Genetic Diversity of 30 Tree Peony (Paeonia suffruticosa Andr.) Cultivars
HOU Xiao-gai,YIN Wei-lun,LI Jia-jue,WANG Hua-fang.AFLP Analysis of Genetic Diversity of 30 Tree Peony (Paeonia suffruticosa Andr.) Cultivars[J].Scientia Agricultura Sinica,2006,39(8):1709-1715.
Authors:HOU Xiao-gai  YIN Wei-lun  LI Jia-jue  WANG Hua-fang
Institution:1, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083; 2,Agricultural College, Henan University of Science and Technology, Luoyang 471003; 3,College of Environmental and Chemical Engineering, Luoyang University, Luoyang 471023
Abstract:【Objective】Investigation of the tree peony's genetic diversity (Paeonia suffruticosa Andr.) is significant in utilizing its germplasm resources and cultivating new varieties. 【Method】The phylogentic relationships among 30 tree peony cultivars were analyzed using AFLP to study the genetic diversity with eight AFLP primer combinations (M+3 and P+3) . 【Result】Among the 1123 bands detected,965 bands were polymorphic and specific bands were found in all 30 tree peony cultivars. The high ratio of polymorphic bands (86%) observed in this study indicate abundant genetic diversity among tree peony cultivars. 【Conclusion】Our results suggest that the 8 pairs of prime combinations could be used to distinguish between all cultivars being tested. Results of cluster analysis showed that the classification based on AFLP markers of cultivars was consistent with their origin.
Keywords:Tree peony(Paeonia suffruticosa Andr  )  Genetic diversity  AFLP analysis
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