| 基因枪法获得转基因小麦植株 |
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| 引用本文： | 侯文胜,郭三堆,路明.基因枪法获得转基因小麦植株[J].中国农业科学,2003,36(5):469-472. |
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| 作者姓名： | 侯文胜 郭三堆 路明 |
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| 作者单位： | 1. 西北农林科技大学农学院/国家小麦改良中心杨凌分中心,杨凌,712100
2. 中国农业科学院生物技术研究所,北京,100081 |
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| 基金项目： | 国家“九五”重点科技攻关资助项目 ( 96 0 0 9 0 1 14 )，杨凌农业生物技术育种中心开放课题资助项目 ( 1999 19) |
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| 摘 要： | 利用适用于禾谷类作物的表达载体 pGU4ABBar,采用基因枪法 ,将人工合成的苏云金芽孢杆菌杀虫晶体蛋白基因cryIa导入小麦品种扬麦 15 8,经PCR和Southernblot鉴定 ,证明获得 4株导入cryIa基因的小麦转基因植株 ,转化率约为 0 .7% ,并通过Westernblot鉴定检测到了目的蛋白的表达。
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| 关 键 词： | 小麦 遗传转化 基因 质粒 蛋白质 |
| 修稿时间： | 2002年1月9日 |
Production of Transgenic Wheat Plants with cryla Gene via Microprojectile Bombardment |
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| HOU Wen sheng ,GUO San dui ,LU Ming.Production of Transgenic Wheat Plants with cryla Gene via Microprojectile Bombardment[J].Scientia Agricultura Sinica,2003,36(5):469-472. |
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| Authors: | HOU Wen sheng GUO San dui LU Ming |
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| Institution: | HOU Wen sheng 1,GUO San dui 2,LU Ming 1 |
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| Abstract: | The gramineous expression vector pGU4ABBar was used in the study. The cryIa gene, a synthetical insecticidal crystal protein gene of Bacillus thuringiensis, had been transferred into wheat variety Yangmai158 by microprojectile bombardment. The PCR and Southern blotting analysis showed that 4 transgenic plants with cryIa gene were obtained. Western blotting analysis had found the aim protein in the transgenic plants was found by western blotting analysis. The transformation frequency was about 0.7%. |
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| Keywords: | Wheat Transformation Gene Plasmid Protein |
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