水稻抗稻瘟病Pib基因的分子标记辅助选择与应用

 【目的】为探索抗稻瘟病Pib基因的分子标记用于水稻抗稻瘟病辅助选育，36个四川地方稻瘟病菌株被用来检测Pib基因的抗性。同时利用检测感病等位基因Pib的显性标记Lys145，并结合前人报道的检测抗病等位基因Pib的显性标记Pibdom组成一套水稻抗稻瘟病基因Pib显性分子标记。【方法】利用这套水稻抗稻瘟病基因Pib显性分子标记对122个杂交稻亲本或材料进行分子鉴定，并分别采用稻瘟病菌株05-12（ZB13）和05-30（ZC15）单小种接种试验进行致病性测试。【结果】所检测的122个杂交稻亲本或材料中，只有7个杂交稻亲本或材料含抗病基因Pib，且对稻瘟病菌菌株ZB13和ZC15表现抗病反应。此外，利用这套水稻抗稻瘟病基因Pib显性分子标记对600个杂交F2代单株进行早期筛选，得到185个抗病基因Pib纯合的单株，田间抗性调查结果与抗病基因分子检测结果一致。【结论】该套显性分子标记可应用于水稻抗稻瘟病基因Pib的分子标记辅助选育。

Marker-assisted Selection and Application of Blast Resistant Gene Pib in Rice

Resistance identifications of pib gene were performed with 36 rice blast fungus strains Collected from Sichuan. Meanwhile the dominant molecular markers of the rice blast resistance gene pib were used to identify the presence in the pib gene of 122 hybrid parents and materials. Pathogenicity assays were performed with the rice blast fungus strains ZB13 and ZC15 with artificial inoculation. The results indicated that pib was a gene of high resistance with a relatively great difference of resistance in different materials. Only 7 of the 122 hybrid parents and materials contained the resistance gene and showed resistance to rice blast fungus strains ZB13 and ZC15. In addition, two pairs of dominant markers Pibdom and Lys145 were used to screen the 6 F2 populations at the early stage. 185 plants of 600 F2 plants containing homozygous pib gene were isolated. At the same time, the rice blast resistances of these plants in the field were investigated. The results demonstrated that the presence of resistance gene consisted with the resistance in the field. Therefore, the set of dominant markers are useful for marker-assisted selection of the rice blast resistance gene pib.