43份三色堇、角堇材料亲缘关系的SRAP分析

【目的】应用SRAP方法分析三色堇和角堇材料的亲缘关系，了解不同材料遗传背景，为品种鉴定和保护以及高效率人工杂交育种和遗传改良提供分子生物学依据。【方法】应用 SRAP ( sequence-related amplified polymorphism) 分子标记方法对43份三色堇、角堇材料进行亲缘关系分析。【结果】从88对引物中筛选出21个多态性较高的引物组合，共产生 500条多态性条带，平均每个引物组合产生23.8条多态性条带，Jaccard’s相似系数在 0.62—0.88。在相似系数为 0.73水平上将 43份材料分为 4个类群，三色堇与角堇明确分开，花色是影响聚类结果的重要因子。【结论】应用SRAP分子标记技术能较准确地解析三色堇和角堇不同材料间的亲缘关系及遗传多样性。

Genetic Relationship of 43 Cultivars of Viola tricolor and Viola cornuta Using SRAP Marker

【Objective】The molecular marker system-SRAP(sequence-related amplified polymorphism) was used to study genetic relationship and investigate genetic backgrounds of Viola tricolor and Viola cornuta for providing a theoretical basis for cultivars identification,intellectual property protection,parent selection and genetic improvement.【Method】 Genetic relationship of 43 Viola tricolor and Viola cornuta cultivars were analyzed using SRAP marker.【Result】 Twenty-one primer pairs selected from 88 primer pairs amplified 500 polymorphic bands with an average of 23.8 polymorphic bands per primer pair.Moreover,Jaccard′s similarity coefficient ranged from 0.62 to 0.88.The 43 cultivars could be divided into 4 groups when the genetic distance was 0.73.Viola tricolor and Viola cornuta could be isolated absolutely.The flower color was a principal factor for the cluster result.【Conclusion】 The genetic relationship and genetic diversity of Viola tricolor and Viola cornuta could be assessed efficiently by SRAP markers.