拟除虫菊酯类农药多残留酶免疫分析方法的建立

 以间苯氧基苯甲酸(PBA)为半抗原，与牛血清蛋白偶联后免疫新西兰大白兔，获得对多种菊酯类农药有特异性的广谱性抗体。以6-氨基己酸法合成PBA与OVA之间含有六碳骨架的桥的偶联物作包被原，建立并优化了间接竞争ELISA检测方法，对PBA最低检出限达0.193 ?g·ml-1，抑制中浓度为3.707 ?g·ml-1，且对氯菊酯、甲氰菊酯、氯氰菊酯、三氟氯氰菊酯、溴氰菊酯均有特异性识别，对氰戊菊酯识别弱，抑制中浓度分别为4.066、4.232、4.286、4.874、8.979、77.360 ?g·ml-1。结果表明，该抗体可用于多种菊酯类农药残留筛选检测。

Development of Enzyme Immunoassays for Pyrethriods

3-phenoxybenzoic acid (PBA) was used as generic hapten in order to generate broad specificity antibody against a group of pyrethriod insecticides. Assay performance was improved by use of a coating antigen, which possessed a six-carbon spacer arm between the hapten and carrier protein. Typical detection limits for PBA was 0.193 ?g·ml-1with an I50 value of 3.707 ?g·ml-1. Different cross-reactions with the closely related pyrethriods,including permethrin, fenpropathrin, cypermetnrin, cyhalothrin and deltamethrin, were observed, with I50 value of 4.066, 4.232,4.286,4.874,8.979 ?g·ml-1, respectively; There was a low cross reactivity for fenvalerate with an I50 value of 77.360 ?g·ml -1. The result developed in this study indicated the antibody was practicable to screen the residues of pyrethriod insecticides.