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| 与马铃薯晚疫病菌无毒基因Avr1连锁的AFLP标记 | |
| 引用本文： | 郭军,Rob Weide,屈冬玉,王晓武,谢开云,Francine Govers.与马铃薯晚疫病菌无毒基因Avr1连锁的AFLP标记[J].中国农业科学,2005,38(9):1801-1804. |
| 作者姓名： | 郭军 Rob Weide 屈冬玉 王晓武 谢开云 Francine Govers |
| 作者单位： | 1. Laboratory of Phytopathology, Wageningen University and Graduate School of Experimental Plant Sciences, Binnenhaven 5, 6709 PD Wageningen,The Netherlands;中国农业科学院蔬菜花卉研究所,北京,100081;西北农林科技大学植物保护学院,杨凌,712100 2. Laboratory of Phytopathology, Wageningen University and Graduate School of Experimental Plant Sciences, Binnenhaven 5, 6709 PD Wageningen,The Netherlands 3. 中国农业科学院蔬菜花卉研究所,北京,100081 |
| 基金项目： | 中-荷联合园艺作物基因组技术实验室资助项目（2001CB711102） |
| 摘 要： | 以具有和不具有无毒基因Avr1表现型的马铃薯晚疫病菌株80029 (AVR1) 和88133 (avr1)以及其杂交F1代50个菌株群体为试材，限制性内切酶PstI和HhaI为酶切组合，采用荧光AFLP技术和混合分组分析法(BSA)，通过256对引物筛选得到了5个与Avr1连锁的候选AFLP标记并进行了菌株个体验证，遗传分析表明5个标记中有2个标记与Avr1连锁且在F1代中共分离，无交换重组发生。 |
| 关 键 词： | 马铃薯晚疫病菌 无毒基因Avr1 BSA AFLP |
| 收稿时间： | 01 27 2005 12:00AM |
| 修稿时间： | 2005-01-27 |
Two AFLP Markers Linked to Avirulence Gene Avr1 in Phytophthora infestans |
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| GUO Jun,Rob Weide,QU Dong-yu,WANG Xiao-wu,XIE Kai-yun,Francine Govers.Two AFLP Markers Linked to Avirulence Gene Avr1 in Phytophthora infestans[J].Scientia Agricultura Sinica,2005,38(9):1801-1804. | |
| Authors: | GUO Jun Rob Weide QU Dong-yu WANG Xiao-wu XIE Kai-yun Francine Govers |
| Institution: | 1.Laboratory ofPhytopathology, Wageningen University, and Graduate School of Experimental Plant Sciences, Binnenhaven 5, 6709 PD Wageningen, The Netherlands; 21nstitute of Vegetables and Flowers, Chinese Academy ricultural Sciences, Beijing 100081; 3College of Plant Protection, Northwest Science and Technology University of Agriculture and Forestry, Yangling 712100 |
| Abstract: | Bulked segregant analysis was used to analyse segregating fluorescent AFLP markers in F1 progeny of Phytophthora infestans cross 71 (NL-80029 X NL-88133) and to select AFLP markers linked to avirulence gene Avr1. All 256 PstI+2 and HhaI+2 primer combinations were screened and this resulted in five candidate AFLP markers linked to Avr1. When tested on 50 individual F1 progenies two out of five candidate AFLP markers appeared to cosegregate with Avr1 and these can be used to position Avr1 on the molecular genetic linkage map. |
| Keywords: | Phytophthora infestans Avr1 BSA (bulked segregant analysis) AFLP marker |
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