| 东乡野生稻苗期耐冷性的QTL定位 |
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| 引用本文： | 夏瑞祥,肖宁,洪义欢,张超,苏琰,张小蒙,陈建民.东乡野生稻苗期耐冷性的QTL定位[J].中国农业科学,2010,43(3):443-451. |
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| 作者姓名： | 夏瑞祥 肖宁 洪义欢 张超 苏琰 张小蒙 陈建民 |
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| 作者单位： | （扬州大学生物科学与技术学院）; |
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| 摘 要： | 【目的】研究东乡野生稻苗期耐冷性的QTL和连锁标记,为水稻耐冷种质资源的利用及分子标记辅助选择育种提供理论和实践依据。【方法】以东乡野生稻作为非轮回亲本,南京11号为轮回亲本,构建144株BC2F1分离群体。通过SSR标记以根电导率作为耐冷性指标,以复合区间定位法对东乡野生稻苗期耐冷性进行QTL定位。【结果】检测到2个QTL qRC10-1和qRC10-2均位于第10染色体,对表型的贡献率分别为34.13%和37.02%,是两个主效的QTL。在与2个QTL的连锁标记RM171周围发展分子标记进一步定位,检测到3个QTL位于标记RM171附近。【结论】东乡野生稻第10染色体上的2个QTLqRC10-1,qRC10-2与苗期耐冷性有关,并位于SSR标记RM304-RM1108区间,可用于水稻耐冷性分子标记辅助选择育种。
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| 关 键 词： | 东乡野生稻 苗期耐冷性 根电导率 QTL定位 |
| 收稿时间： | 2009-03-04; |
QTLs Mapping for Cold Tolerance at Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon Griff.) |
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| XIA Rui-xiang,XIAO Ning,HONG Yi-huan,ZHANG Chao,SU Yan,ZHANG Xiao-meng,CHEN Jian-min.QTLs Mapping for Cold Tolerance at Seedling Stage in Dongxiang Wild Rice (Oryza rufipogon Griff.)[J].Scientia Agricultura Sinica,2010,43(3):443-451. |
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| Authors: | XIA Rui-xiang XIAO Ning HONG Yi-huan ZHANG Chao SU Yan ZHANG Xiao-meng CHEN Jian-min |
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| Institution: | XIA Rui-xiang1,XIAO Ning2,HONG Yi-huan1,ZHANG Chao1,SU Yan1,ZHANG Xiao-meng1,CHEN Jian-min1 (1College of Bioscience , Biotechnology,Yangzhou University,Yangzhou 225009,Jiangsu,2Lixiahe Region Agricultural Research Institute of Jiangsu/Nanjing Sub-Center of National Rice Improvement Center,Yangzhou 225000,Jiangsu) |
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| Abstract: | Objective The objective of the study is to identify quantitative trait loci (QTLs)controlling cold tolerance at seedling stage and linkage SSR markers in Dongxiang wild rice. Method Cross was made between cold tolerant Dongxiang wild rice (Oryza rufipogon Griff.) and cold susceptible rice variety Nanjing11. Nanjing 11 was then used as the recurrent parent to construct a backcross segregation population (BC2F1) which includes 144 plants. This population was used to detect QTLs for cold tolerance at seedling ... |
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| Keywords: | Dongxiang wild rice cold tolerance at seedling stage root conductivity QTL mapping |
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