TrxS基因的表达及其对大麦种子淀粉酶活性的影响

 【目的】分析TrxS基因对大麦种子发芽及淀粉酶活性的影响,为TrxS基因对大麦种子代谢的调控提供了理论依据。【方法】以转TrxS基因啤酒大麦株系为材料，从分子水平和生理指标进行分析。【结果】实时荧光RT-PCR检测证明TrxS基因在RNA水平上能够表达；转基因大麦籽粒α-淀粉酶和β-淀粉酶活性明显提高，发芽1-5d转基因大麦种子α-淀粉酶活性比对照分别增加5.86%、75.24%、118.51%、71.02%和22.99%，β-淀粉酶活性分别比对照增加19.29%、14.68%、35.47%、23.62%和2.67%；种子的发芽能力提高。【结论】TrxS基因能够促进种子发芽。

Expression of TrxS and its Effect on Amylase Activities in Barley Seeds

The paper analyzed the expression of TrxS gene and its effect on amylase activities of barley seeds during germination period, the results showed that TrxS gene could express in transgenic barley seeds by the detection of real-time PCR. During germinating days 1-5d, the activities ofα- amylase were 5.86%、75.24%、118.51%、71.02%、22.99% higher than that of none-transgenic seeds, respectively; the activities ofβ- amylase were 19.29%、14.68%、35.47%、23.62%、2.67% higher than that of none-transgenic seeds, respectively; the germination vigor of transgenic seeds was higher than that of none-transgenic seeds, either. The results provided theoretical basis for the regulation of seed metabolism.