水稻叶蝉抗性基因回交转育和CAPS标记辅助选择

 以综合性状好但对黑尾叶蝉 (NephotettixcincticepsUhler)敏感的品种台中 6 5作为轮回亲本 ,与抗性品种DV85连续回交 ,得到回交高代BC6F2 群体 ,进行抗叶蝉性状的回交转育。将抗黑尾叶蝉基因Grh2两侧的RFLP标记C189和G14 6 5成功地转换为在亲本间具有多态的CAPS标记。在进行表型选择的同时 ,利用CAPS标记对BC6F2 进行了标记辅助选择 ,分析了CAPS标记与Grh2间的遗传距离和标记辅助选择的效果。所选出的个体具有台中 6 5的遗传背景且携带纯合Grh2基因 ,可作为聚合抗叶蝉基因培育新品种的重要中间材料。

Green Rice Leafhopper Resistance Gene Transferring Through Backcrossing and CAPS Marker Assisted Selection

In order to transfer the resistance to green rice leafhopper (Nephotettix cincticeps Uhler) into Taichung65, a japonica cultivar with elite characters, the resisitant indica cultivar DV85 was backcrossed with Taichung65 as the recurrent parent. Grh2, one of resistance genes was located on chromosome 11 of resistant variety DV85. C189 and G1465, two RFLP markers flanking Grh2 gene, were transformed into CAPS markers. Both phenotypic selection and CAPS marker assistant selection were conducted in the BC 6F 2 population derived from the cross of Taichung65 and DV85 to pick out the important breeding materials with Taichung65 background and resistance to green rice leafhopper. The linkage distance was calculated with the molecular and phenotypic data, meanwhile the effect of the selection method was analyzed.