绿豆抗豆象基因PCR标记的构建与应用

 采用PCR分子标记技术，对16个绿豆品种（系）进行了遗传分析。在选用的56个随机引物中，发现抗豆象品种与感豆象品种间有一定差异。根据聚类分析结果将它们分成抗豆象野生种（TC1966）、抗豆象栽培种（V2709）、抗豆象杂交后代（VC3890A2/TC1966-23）和混合类型4个大组。以绿豆抗豆象和感豆象品种及抗豆象品种×感豆象品种组合的F2群体为试验材料，利用BSA法，对抗（感）豆象品种池和一个组合F2的抗（感）豆象池进行了鉴定，获得一个共显性标记。经F2分析，在抗豆象个体中扩增出约1.79 kb的特异片段或2个特异片段（1.79 kb/1.03 kb）；在感豆象个体中仅扩增出约1.03 kb的特异片段。初步认为此标记与TC1966的抗豆象基因位点紧密联锁，可用于绿豆抗豆象种质鉴定和遗传育种的分子标记辅助选择。

Tagging and Utilization of Bruchid Resistance Gene Using PCR Markers in Mungbean

Polymerase chain reaction (PCR) conditions suitable for discriminating mungbean bruchid resistance genotypes were determined. Sixteen accessions were examined with 56 random primers which produced different PCR bands among them. These mungbean accessions can be classified into 4 groups, i. e., the wild-type resistance group (TC1966), the cultivar resistance group (V2709), the progeny resistance group(VC3890A2/TC1966-23) and the susceptible varieties with their progenies group. Mungbean cultivars with bruchid resistance or susceptibility and their crosses F2 of bruchid resistance wild-type with susceptible cultivar were used as materials in this experiment. By using BSA method, two codominant PCR markers were identified through the resistant (susceptible) bruchid cultivars bulks and the resistant (susceptible) bruchid bulks of a F2 population. There was a 1.03 kb band in the susceptible individuals and a 1.79 kb or two 1.79 kb/1.03 kb bands in the resistant individuals. It is sreculated that the markers are closely linked with TC1966 bruchid resistance/ susceptible elleles, and they can be applied in bruchid resistance identification of the mungbean and markers-assisted selection in bruchid resistance breeding of mungbean.