| 高效液相色谱-电喷雾质谱联用法分析罗汉果皂甙 |
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| 引用本文： | 戚向阳,张俐勤,单夏锋,陈维军,宋云飞.高效液相色谱-电喷雾质谱联用法分析罗汉果皂甙[J].中国农业科学,2005,38(10):2096-2101. |
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| 作者姓名： | 戚向阳 张俐勤 单夏锋 陈维军 宋云飞 |
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| 作者单位： | [1]华中农业大学食品科技学院,武汉430070 [2]中国农业科学院茶叶研究所,杭州310008 [3]桂林莱茵生物制品有限公司,桂林541300 |
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| 基金项目： | 华中农业大学创新基金. |
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| 摘 要： | 【目的】建立罗汉果皂甙的HPLC/ESI/MS2定性分析方法。【方法】采用高效液相色谱-电喷雾质谱联用法对罗汉果皂甙提取物进行分离分析。【结果】根据主要色谱峰的质谱特征，初步鉴定出罗汉果皂甙提取物中主要的皂甙成分为11-氧-罗汉果皂甙V（11-oxo-mogroside-V）、罗汉果皂甙V（mogroside-V）、罗汉果皂甙IV（mogroside-IV）、罗汉果皂甙VI（mogroside-VI）和赛门甙I（siamenoside-I）。【结论】对于罗汉果皂甙的分离及定性分析，高效液相色谱-电喷雾质谱(HPLC／ESI/MS2)联用分析法是一种方便快捷有效的方法。
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| 关 键 词： | 罗汉果(Siratia grosvenori) 罗汉果皂甙 高效液相色谱-电喷雾质谱联用法 |
| 收稿时间： | 10 19 2004 12:00AM |
| 修稿时间： | 2004-10-19 |
Study on Analysis of Mogrosides by HPLC/ESI/MS2 |
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| QI Xiang-yang, ZHANG Li-qin, SHAN Xia-feng, CHEN Wei-jun, SONG Yun-fei.Study on Analysis of Mogrosides by HPLC/ESI/MS2[J].Scientia Agricultura Sinica,2005,38(10):2096-2101. |
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| Authors: | QI Xiang-yang ZHANG Li-qin SHAN Xia-feng CHEN Wei-jun SONG Yun-fei |
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| Institution: | 1.Department of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070; 2.Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008; 3. Guilin Laiyin Natural Ingredients Corporation, Guilin 541300 |
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| Abstract: | 【aim】A HPLC/ESI/MS2 method was developed for the qualitative analysis of mogrosides from fruits of Siratia grosvenori.【method】HPLC/ESI/MS2 was used to separate and analyze mogroside extracts from fruits of Siratia grosvenori. 【result】The main components of mogroside in mogroside extracts were 11-oxo-mogroside-V, mogroside-V, mogroside-IV, mogroside-VI and siamenoside-I according to mass spectrometry analysis of main chromatogram peaks. 【conclusion】HPLC／ESI/MS2 is a convenient, rapid and effective method for separation and qualitatively analysis of mogrosides from fruits of Siratia grosvenori. |
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| Keywords: | Siratia grosvenori Mogrosides HPLC/ESI/MS^2 |
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