精子抗原基因在猪生殖道及成熟精子中的表达特性

【目的】揭示若干精子抗原基因(SPAG1、SPAG5、SPAG6、SPAG11C和SPAG11E)在梅山公母猪生殖道及成熟精子中的mRNA时空表达规律。【方法】利用RT-PCR技术分析各基因在生殖道和精子中组织表达谱,利用半定量RT-PCR分析其在初生至150d梅山公猪睾丸中的发育性表达特性。【结果】5个基因在梅山公母猪生殖道组织中呈现不同的表达特性。其中SPAG1在睾丸中表达丰度最高,在精囊腺、前列腺、附睾体、子宫角和输卵管呈现中等丰度表达,而在附睾尾、子宫颈和卵巢的表达信号较弱。SPAG5在睾丸中表达丰度最高,在前列腺、附睾体和子宫颈中表达信号较弱。而SPAG6在睾丸中高丰度表达,在输卵管中呈现中等丰度表达,在子宫角的表达信号很弱。SPAG11C在附睾体和睾丸中高水平表达,而SPAG11E则在附睾体和附睾尾中低水平表达。在成熟精子中只有SPAG11E有mRNA表达。SPAG1、SPAG5、SPAG6和SPAG11C基因在公猪睾丸中发育性表达分析表明,总体上所检测的4个精子抗原基因的表达水平都随着日龄增加而提高,但存在一些差异。其中SPAG1和SPAG11C呈现相似的表达规律,在初生和30日龄时表达相对较低,但在60、90、150日龄都有显著提高(P0.05)。而SPAG5在初生和30日龄时表达也相对较低,在60和90日龄时均有显著提高(P0.05),但150日龄时略有下降,但差异不显著。SPAG6在初生和30日龄时表达水平很低,至60日龄有显著提高(P0.05),90日龄时维持这一水平,而到150日龄时则有显著下降(P0.05)。【结论】SPAG1、SPAG5、SPAG6、SPAG11C和SPAG11E在梅山公母猪生殖道组织广泛表达,在睾丸组织中其mRNA表达水平变化与性发育相一致,SPAG11E在精子中存在低丰度mRNA表达。

Characterized of the Spatial and Temporal Expression of Several Sperm-Associated Antigen Genes in the Genital Tracts and Mature Sperm of Pigs

【Objective】 The aim of current study is to investigate the spatial and temporal expression profiles of several sperm-associated antigen genes (SPAG, SPAG5, SPAG6, SPAG11C and SPAG11E) in the genital tracts and mature sperm of Meishan boars and gilts. 【Method】 The RT-PCR technique was used to detect the spatial expression patterns of these genes in genital tracts and sperm, while semi-quantitative RT-PCR was used to examine the temporal expression of each gene in testis. 【Result】 Five genes presented different expression patterns in the genital tracts of Meishan boars and gilts, and SPAG1 gene expression was the highest in testis, moderate in seminal vesicle, prostate, corpus epididymis, uterine horn and oviduct, weak in cauda epididymis, cervix and ovary. SPAG5 expressed mainly in testis and weakly in prostate, corpus epididymis and cervix. While the expression of SPAG6 was the highest in testis, moderate in oviduct and weak in uterine horn. SPAG11C expressed highly in corpus epididymis and testis, whereas SPAG11E expressed weakly in corpus and cauda epididymis. Only SPAG11E mRNA expression in mature sperm was detected. The RT-PCR analysis of the temporal expression of SPAG, SPAG5, SPAG6 and SPAG11C genes in testis revealed that, in general, the expression of all genes examined elevated with age increase but with some variance. The expression of SPAG1 and SPAG11C presented similar trend, and it was low on days 1 and 30, but it increased significantly on days 60, 90 and 150 (P＜ 0.05). The mRNA expression of SPAG5 was relatively low on days 1 and 30 as well, but increased significantly on days 60 and 90 (P＜0.05), then decreased slightly on day 150. The mRNA expression of SPAG6 was low on days 1 and 30, increased significantly on day 60 (P＜0.05) and maintained to day 90, but decreased significantly on day 150 (P＜0.05). 【Conclusion】 The expression of SPAG, SPAG5, SPAG6, SPAG11C and SPAG11E in genital tracts was more extensive, weak expression of SPAG11E was detected in mature sperm, and the temporal expression of these genes was paralleled with the sexual development.