秦川牛及其杂种牛POU1F1基因多态与生长性能相关性

 本研究首次利用PCR-RFLP技术研究相同季节4月（±10 d）龄纯种秦川牛（QQ）及杂种牛秦安（AQ）、秦德（DQ）、秦利(LQ) 4个群体164个个体POU1F1基因多态性及其与体重、体尺等生长性状指标之间的相关性。结果表明， QQ及AQ、DQ、LQ群体POU1F1-HinfI基因座的451 bp 的PCR产物被限制性酶HinfI消化后表现多态性，它们的等位基因A/B频率分别为：0.232/0.768、0.333/0.667、0.178/0.822和0.181/0.819，且均处于Hardy-Weinberg平衡状态。同时，4个群体POU1F1基因座不同基因型与体重、体尺等生长性状指标相关分析的结果表明，4个群体内AB、BB基因型个体在胸围、十字部高指标上显著高于群体AA型个体（P＜0.05），即BB、AB＞AA（P＜0.05），可作为秦川牛体尺指标(胸围、十字部高)候选基因之一。但在体长指标上均无显著差异（P＞0.05），所以不宜作为体长指标候选基因。初步认为BB基因型为优势基因型，相应地B为优势等位基因，对选择有正向效应。

Correlation of Polymorphisms of POU1 F1 Gene and Growth Traits in Qinchuan Cattle and Its Hybrid Cattle

PCR-RFLP was firstly applied to analyze the correlation of polymorphisms of POU1F1 gene and growth traits in 164 beef cattle, at the age of 4 months (±10 d), including Qinchuan cattle, Qinchuan×Limousin cattle, Qinchuan×Red Angus and Qinchuan×German Yellow cattle. The results showed that 451 bp PCR products digested with HinfI demonstrated polymorphisms in four populations which were at Hardy-Weinberg equilibrium (P>0.05). Moreover, the frequencies of allele A/B of QQ, AQ, DQ and LQ populations were 0.232/0.768, 0.333/0.667, 0.178/0.822 and 0.181/0.819, respectively. The correlation of polymorphisms of POU1F1 gene and growth traits among the 4 populations was analyzed. In heart girth, height at hip cross, individuals with genotype AB and BB in population were higher than that of genotype AA, that is, BB and AB＞AA (P＜0.05). So POU1F1 gene can be one of a candidate genes for growth traits with heart girth, height at hip cross, but not for body length trait. The conclusion is that genotype BB is a predominant genotype and B is a predominant allele.