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| 柱前衍生高效液相色谱法测定果蔬产品阿维菌素及其有毒代谢物的残留量 | |
| 引用本文： | 谢显传,张少华,王冬生,皇甫伟国,杨挺,何新华.柱前衍生高效液相色谱法测定果蔬产品阿维菌素及其有毒代谢物的残留量[J].中国农业科学,2005,38(11):2254-2260. |
| 作者姓名： | 谢显传 张少华 王冬生 皇甫伟国 杨挺 何新华 |
| 作者单位： | 1. 上海市农业科学院植物保护研究所,上海市设施园艺技术重点实验室,上海,201106 2. 宁波市农业科学院,宁波,315040;广西大学农学院,南宁,530004 3. 宁波市农业科学院,宁波,315040 4. 广西大学农学院,南宁,530004 |
| 基金项目： | 上海市科技兴农重点攻关项目（沪农科攻字2003第10-6号） |
| 摘 要： | 建立了快速测定果蔬产品中阿维菌素及其有毒代谢物残留量的柱前衍生高效液相色谱测定方法。果蔬样品经乙腈提取后，直接采用三氟乙酸酐(TFAA)－N－甲基咪唑(NMIM)－乙腈(ACN)法进行柱前荧光衍生化，后以高效液相色谱-荧光检测器进行阿维菌素残留分析。试验结果表明，本方法对果蔬产品中的阿维菌素及其有毒代谢物残留量的最低检测浓度为1μg·kg-1，在0.001～0.1mg·kg-1时，阿维菌素的添加回收率在84.2%～110.8%之间，变异系数（C.V.%）在1.9%～12.8 %。 |
| 关 键 词： | 阿维菌素 衍生 残留 高效液相色谱 蔬菜 水果 |
| 收稿时间： | 07 19 2005 12:00AM |
| 修稿时间： | 2005-07-19 |
Determination of Abamectin and Its Toxicological Metabolite in Vegetables and Fruits by HPLC with Pre-column Fluorescent Derivatization |
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| XIE Xian-chuan,ZHANG Shao-hua,WANG Dong-sheng,HUANG PU Wei-guo,YANG Ting,HE Xin-hua.Determination of Abamectin and Its Toxicological Metabolite in Vegetables and Fruits by HPLC with Pre-column Fluorescent Derivatization[J].Scientia Agricultura Sinica,2005,38(11):2254-2260. | |
| Authors: | XIE Xian-chuan ZHANG Shao-hua WANG Dong-sheng HUANG PU Wei-guo YANG Ting HE Xin-hua |
| Institution: | XIE Xian-chuan1,ZHANG Shao-hua2,3,WANG Dong-sheng1,HUANG PU Wei-guo2,YANG Ting2,HE Xin-hua3 |
| Abstract: | A rapid HPLC method for simultaneous determination of abamectin and its toxicological metabolite in vegetables and fruits with fluorescent detector after pre-column derivatization was developed. After extracted by acetonitrile, overleaping cleaned-up step,the target compounds in samples was directly pre-column derivatised by TFAA-NMIM-CAN system. The results suggested that the fortified recovery and variation coefficient of added compouds in samples were 84.2%～110.8% and 1.9%～12.8%, respectively, the limited level of detection is 1μg·kg-1. |
| Keywords: | Abamectin Derivatization Residues HPLC Vegetable Fruit |
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