骨蛋白水解物抗氧化活性及其作用模式

 【目的】研究骨蛋白水解物的抗氧化活性及其抗氧化作用模式。【方法】将骨蛋白用碱性蛋白酶水解0.5～6 h，测定骨蛋白水解物的还原能力、金属螯合能力、清除自由基能力来综合分析骨蛋白水解物的抗氧化作用模式。【结果】骨蛋白水解物的自由基清除能力、Cu2+和Fe2+螯合能力以及还原能力均随水解时间的延长而增大，而且抗氧化活性随着水解物浓度的增加而增加。底物浓度为7％、水解5 h的骨蛋白水解物与其它水解条件下的水解物相比，具有较高的抗氧化活性和较强的清除自由基能力，且具有丁基羟基茴香醚（butylated hydroxyanisole，BHA）、没食子酸丙酯（proply gallate，PG）等抗氧化剂所不具备的金属螯合能力。7%的骨蛋白水解物与0.02% PG的抗氧化能力相近。尽管未水解的骨蛋白也有一定的抗氧化性，但其活性远低于骨蛋白水解物。【结论】骨蛋白水解物具有较高的抗氧化活性，可以作为金属离子螯合剂、氢供体和自由基稳定剂来抑制脂肪氧化。

Antioxidant Activity and the Possible Mode of Action of Bone Protein Hydrolysates

【Objective】 Antioxidant activity of bone protein hydrolysates and mode of antioxidative action were studied in this paper. 【Method】Bone protein was hydrolyzed for 0.5-6 h by alcalase. The mode of antioxidative action was evaluated by reducing power, metal-chelation ability and radical-scavenging. 【Result】 Bone protein hydrolysates possessed strong Cu2+- and Fe2+-chelation ability and marked reducing power, both of which were increased with hydrolysis time. The protein hydrolysates also showed strong radical-scavenging ability, and the antioxidant activity increased with substrate concentration. The antioxidant activity of 7% alcalase-hydrolyzed bone protein was comparable to those of butylated hydroxyanisole and proply gallate, and its antioxidant activity was close to proply gallate. Although intact bone protein displayed an antioxidative effect, it was far less potent than hydrolyzed bone protein. 【Conclusion】 The results demonstrated that enzyme-hydrolyzed bone protein can act as a metal ion chelator, a hydrogen donor, as well as a radical stabilizer to inhibit lipid oxidation.