应用ISSR分子标记和表型性状评价孔雀草自交系的遗传关系

 【目的】对亲本遗传距离的评估是保证F1代种子杂种优势的基础工作，本研究的目的是为科学的选择孔雀草杂交育种亲本奠定基础。【方法】对12份孔雀草自交系和4份孔雀草F1代及两份万寿菊材料分别用ISSR分子标记和形态学特征进行了遗传分析。【结果】用10个引物共得到145个位点，其中138个为多态性位点；对ISSR标记和10个表型性状分别进行分析的结果表明，当优先考虑花型和材料来源两个表型性状时，两种分类方法得到的结果基本一致。【结论】在孔雀草分类中以花型和材料来源为重要依据时，能够比较客观地反映其遗传基础上的差异。在孔雀草利用杂种优势育种中，选择花型差异大，来源不同的亲本，如孔雀草nana和孔雀草黄色小英雄，孔雀草nana Petite Gold和孔雀草21605，有可能得到性状优良的杂交后代。

Evaluation of Genetic Relationships of Tagetes patula Inbred Lines Using ISSR Markers and Morphological Traits

【Objective】Assessment of genetic distance of the parents was essential work to ensure the heterosis of F1 seeds. In order to select the right lines for crossing of Tagetes plant, 【Method】the genetic relationship of 18 inbred lines of Tagetes was analyzed with ISSR markers and morphological traits. 【Result】With 10 ISSR primers, 138 polymorphic loci were detected out of 145 loci in total. Although the results obtained by ISSR and morphological traits were generally similar, there were some differences. 【Conclusion】ISSR markers could provide genetic basis for select inbred lines as crossing parents of Tagetes according to the genetic distances.