|
|||||
|
|
| 中红侧沟茧蜂非典型气味受体的克隆及组织特异性表达 | |
| 引用本文： | 张帅,张永军,苏宏华,高希武,郭予元.中红侧沟茧蜂非典型气味受体的克隆及组织特异性表达[J].中国农业科学,2009,42(5):1639-1645. |
| 作者姓名： | 张帅 张永军 苏宏华 高希武 郭予元 |
| 作者单位： | 1. 中国农业科学研究院植物保护研究所/植物病虫害生物学国家重点实验室,北京,100193;中国农业大学农学与生物技术学院,北京,100193 2. 中国农业科学研究院植物保护研究所/植物病虫害生物学国家重点实验室,北京,100193 3. 中国农业大学农学与生物技术学院,北京,100193 |
| 基金项目： | 国家自然科学基金重点项目 |
| 摘 要： | 【目的】研究中红侧沟茧蜂Microplitis mediator非典型气味受体的表达谱。【方法】使用RT-PCR法克隆得到中红侧沟茧蜂非典型气味受体基因序列。使用实时荧光定量PCR法研究其表达谱。【结果】克隆得到中红侧沟茧蜂非典型气味受体全长基因。该基因在触角中特异性表达。在雌虫触角中羽化当天达到最大表达量，雄虫触角中在羽化前d阶段达到最大表达量。【结论】该非典型气味受体在中红侧沟茧蜂触角中特异存在。 |
| 关 键 词： | 中红侧沟茧蜂 嗅觉 非典型气味受体 组织表达 |
| 收稿时间： | 2008-5-29 |
Gene Cloning and Tissue-Specific Expression of an Olfactory Receptor in Microplitis Mediator (Hymenoptera: Braconidae) |
|
| ZHANG Shuai,ZHANG Yong-jun,SU Hong-hua,GAO Xi-wu,GUO Yu-yuan.Gene Cloning and Tissue-Specific Expression of an Olfactory Receptor in Microplitis Mediator (Hymenoptera: Braconidae)[J].Scientia Agricultura Sinica,2009,42(5):1639-1645. | |
| Authors: | ZHANG Shuai ZHANG Yong-jun SU Hong-hua GAO Xi-wu GUO Yu-yuan |
| Institution: | Institute of Plant Protection, Chinese Academy of Agriculture Science |
| Abstract: | 【Objective】 The expression of an olfactory receptor of Microplitis mediator was studied. 【Method】 The olfactory receptor gene of Microplitis mediator was cloned using RT-PCR. And its tissue-specific expression and quantitative changes at different developmental stages were detected using real-time PCR. 【Result】 A gene encoding a novel olfactory receptor, which is antenna- specific, was isolated from Microplitis mediator (Hymenoptera: Braconidae). Its expression levels reached the maximum amount on the emergence day of female and reached the maximum amount at the d stage before emergence of male. 【Conclusion】This receptor is antenna- specific in Microplitis mediator. |
| Keywords: | Microplitis mediator olfaction olfactory receptor expression pattern |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《中国农业科学》浏览原始摘要信息 | |
| 点击此处可从《中国农业科学》下载免费的PDF全文 | |