小麦品种N.Strampelli的抗条锈基因定位与分子作图

 【目的】N.Strampelli是一个十分重要的持久抗源材料,研究其抗病性遗传特点,抗条锈病基因的定位与分子作图,对揭示品种持久抗病性遗传机制,科学有效利用该优质抗源材料选育持久抗病性品种具有重要意义。【方法】将N.Strampelli分别与铭贤169和中国春杂交、回交并对双亲及其杂交后代进行遗传分析。以中国春单体系作母本分别与N.Strampelli杂交获得经镜鉴的F1代,F1代套袋自交获得F2代单体材料并进行抗病基因染色体定位。用于遗传分析和单体定位的小麦条锈菌为SU-4、CYR31、CYR29-mut3。选用普通小麦的208对SSR分子标记对N.Strampelli及铭贤169的基因组DNA进行PCR扩增和电泳分析。【结果】N.Strampelli对SU-4菌系的抗病性由2对隐性基因重叠或独立控制;对CYR31菌系的抗病性由2对隐性基因互补控制;对CYR29-mut3的抗病性由一对隐性核基因控制,并将该基因暂命名为YrN.S。建立了与YrN.S连锁的3个微卫星标记Xgwm499、Xwmc415、Xwmc537,其与YrN.S的遗传距离分别为7.6、5.4和10.7cM,将YrN.S定位于小麦5BL上。【结论】YrN.S是一个与已知抗条锈病基因不同的新基因。

Molecular Mapping and Monosomic Location of Stripe Rust Resistant Gene in N.Strampelli

【Objective】 N. Strampelli is a very important source of durable resistant material. Inheritance, location and molecular mapping of stripe rust resistance genes in N. Strampelli is important for revealing the resistance to stripe rust and developing durable cultivars. 【Method】 To confirm the resistant genes, N. Strampelli was crossed with susceptible cultivar MingXian169, Chinese spring and then backcrossed with them. The gene’s chromosome location was determined by the monosomic analysis of Chinese spring, which were then backcrossed among them. The microscope observation of F1 was performed to assess whether they were monosomic materials. To test molecular markers for N. Strampelli, 208 SSR pairs of primers, the PCR amplification, non-denatured PAGE and silver staining are used for detecting polymorphic bands. 【Result】 The resistance to SU-4 was dependently or overlappedly controlled by two recessive genes. The resistance to CYR31 was overlappedly controlled by two recessive genes. There was one recessive resistant gene controlled when tested with CYR29-mut3. It was temporarily named YrN.S. Xgwm499, Xwmc415 and Xwmc537 were located on chromorome 5BL. The map distance between Xgwm499, Xwmc415 and Xwmc537 were 7.6 cM, 5.4 cM and 10.7 cM, respectively. 【Conclusion】 The result indicated that YrN.S is a novel resistant gene which is different from other stripe rust resistance genes.