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我国棉花黄萎病菌群体的遗传变异分析
引用本文:周兆华,郭旺珍,潘家驹,张天真.我国棉花黄萎病菌群体的遗传变异分析[J].中国农业科学,1999,32(2):60-65.
作者姓名:周兆华  郭旺珍  潘家驹  张天真
作者单位:南京农业大学农学系,农业部农作物种质资源与育种重点开放实验室,南京,210095
基金项目:国家“九五”科技攻关,国家发展棉花生产专项基金,国家教委博士点基金
摘    要: 对源于我国主要棉区的24个棉花大丽轮枝菌株,美国的T9和一个从茄子植株上分离的大丽轮枝菌株进行了性状培养观察、致病性测定及遗传多样性分析。结果表明,用中选的25个随机引物对26个菌株进行RAPD-PCR扩增,产生379条DNA带,其中223条为多态性带;对26个菌株的RAPD指纹图谱聚类分析可将其分为8大类,聚类结果与其地理来源相关性不大,而与菌株的致病性程度有很大的相关性;落叶型和非落叶型菌株的遗传基础差异很大。通过不同菌株对棉花品种的田间接种试验,依其致病程度区分,结果与RAPD指纹图谱聚类的结果基本吻合。

关 键 词:棉花  黄萎病  大丽轮枝菌  遗传多样性  致病力
修稿时间:1998-03-18

Analyses on the Genetic Variation of Cotton Verticillium dahliae Isolate Populations in China
Zhou Zhaohua,Guo Wangzhen,Pan Jiaju,Zhang Tianzhen.Analyses on the Genetic Variation of Cotton Verticillium dahliae Isolate Populations in China[J].Scientia Agricultura Sinica,1999,32(2):60-65.
Authors:Zhou Zhaohua  Guo Wangzhen  Pan Jiaju  Zhang Tianzhen
Abstract:cotton Verticillium dahliae isolates from the main cotton growing regions of China, one T9 isolate from U.S.A. and another one from eggplant were cultured for study on their characteristics. Their virulences were determined and genetic polymorphisms were analyzed. Results indicated that when the 26 isolates were amplified by RAPDPCR with 25 random primers, 379 RAPD bands were identified among which 233 were polymorphic bands. The 26 isolates were classified into eight groups by RAPD finger printing clustering analyses. Clustering results kept no close correlation with their geographical origin, but showed close correlation with the virulence degree. Defoliating types had significant genetic diversification with nondefoliating types. According to virulence degree, the classification of isolates through field tests was fundamentally in line with classification by RAPD finger printing clustering analyses.
Keywords:Cotton  Verticillium wilt  Verticillium    dahliae  Genetic polymorphism  Pathogenicity  
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