ALV-J相关的鸡急性纤维肉瘤发病模型的建立

【目的】证明纤维肉瘤病料中含有的ALV-J相关病毒能够诱发急性纤维肉瘤。【方法】将含ALV-J相关病毒的病料过滤液经不同部位接种1日龄817肉杂鸡；将该过滤液不同倍数稀释后接种1日龄SPF鸡和817肉杂鸡；将该过滤液接种细胞培养后的上清液接种1日龄817肉杂鸡，连续动态观测30 d。【结果】颈部皮下、胸肌、腹腔3个部位攻毒鸡的肿瘤发生率分别为100%（20/20）、95.2%（20/21）、100%（20/20）。病料过滤液未经稀释接种817肉杂鸡和SPF鸡的肿瘤发生率均为100%（10/10），10倍稀释后攻毒鸡的肿瘤发生率分别为80%（8/10）和100%（10/10），100倍稀释后攻毒鸡的肿瘤发生率分别为100%（10/10）和80%（8/10），1 000倍稀释后攻毒鸡的肿瘤发生率分别为40%（4/10）和50%（5/10），更高倍数稀释后攻毒鸡没有肿瘤发生。接种病料的细胞培养上清也能使33.3%（2/6）的鸡只发生肉瘤。病理组织学检测显示：诱发的急性肿瘤系典型的纤维肉瘤。【结论】817肉杂鸡肉瘤病料中含有ALV-J相关的急性致肿瘤病毒，不论是病料过滤液本身还是其接种DF-1细胞培养后的上清液都能在不同品种鸡复制出急性纤维肉瘤，最快在接种后7 d出现，且肿瘤发生的时间、动态和肿瘤发生率与接种量密切相关。

Establishment of a Bird Experiment Model for Chicken Acute Fibrosarcomas Induced by ALV-J Associated Virus

【Objective】 An experiment was carried out to prove that the field chicken fibrosarcoma mass contains acute fibrosarcoma-inducing ALV-J associated virus.【Method】 One-day-old 817 hybrid chickens were inoculated through different routes with tumor suspension filtrate containing ALV-J associated virus.One-day-old 817 hybrid chickens and SPF Leghan chickens were inoculated subcutaneously with tumor suspension filtrate containing ALV-J associated virus in different doses.Continuous observation of tumor development was made in 30 days.【Result】 In the groups of inoculations through the subcutaneous,in muscule and intra-abdominal,the tumor incidence was 100%(20/20),95.2%(20/21),and 100%(20/20),respectively.The tumor incidence of 817 hybrids and SPF chickens inoculated with undiluted tumor extract filtrate was 100%(10/10).When birds were inoculated with tumor extracts in 1﹕10 and 1﹕100,tumor rates were still in the range of 80%(8/10) to 100%(10/10) for both chicken lines.When birds were inoculated with tumor extracts in 1﹕1 000,tumor rates decreased to 40%(4/10) or 50%(5/10).No tumor development was observed in 30 days when birds were inoculated with the tumor extract of 1﹕10 000 dilution.However,there were 2/6 chickens developed tumor mass when inoculated with supernatant from tumor extract-infected DF-1 cell culture.Histopathology indicated that the induced tumors were typical fibrosarcoma.【Conclusion】The fibrosarcoma tissues of 817 hybrid chickens of field case contained ALV-J associated acutely tumorigenic virus.Both tumor extract filtrate or extract-infected DF-1 cell culture supernatant could induce acute fibrosarcomas in chickens of different lines,and the tumor started to appear as early as 7 days after inoculation.The dynamics of the fibrosarcoma development and tumor rates were closely related to doses of the tumor extracts in inoculation.