临床型奶牛乳房炎乳腺组织的比较蛋白质组研究

 【目的】采用比较蛋白质组学技术分析、检测了临床健康与乳房炎奶牛乳腺组织中的差异表达蛋白，以寻找药物治疗目标，探讨奶牛乳房炎的发生机理。【方法】采用二维凝胶电泳分离临床健康与乳房炎奶牛的乳腺组织蛋白，考马斯亮蓝染色，PDQuest7.4软件自动匹配检测差异表达蛋白斑点，高效液相色谱串联离子阱质谱分析鉴定。【结果】凝胶图谱中检测出15个差异蛋白斑点，串联质谱分析后有12个蛋白斑点可搜索到相应的结果，对应于10种蛋白质，主要涉及结合、运输和催化活性等分子功能，参与细胞、代谢及生物调节等生理过程。【结论】临床健康与乳房炎奶牛的乳腺组织中蛋白的表达模式存在差异，表达差异蛋白与奶牛乳房炎的发生相关，对其分析有利于探索机体的生理病理状态，还可为揭示奶牛乳房炎的发生机理提供直接依据。

Comparative Proteome Analysis of Changes in Clinical Healthy and Mastitic Cows Mammary Gland Expression Protein

【Objective】 Changes of protein expression were investigated in mammary gland from healthy dairy cows and clinical mastitic cows in order to detect the disease-related proteins and understand molecular mechanism of clinical mastitis at the protein level. 【Method】 The project presents the protein changes in mammary gland using two-dimensional gel electrophoresis (2-DE), after stained with colloidal coomassie blue, differential expression proteins were detected by PDQuest 7.4 software and subjected to ion trap mass spectrometer equipped with a Surveyor HPLC system, differential spots of protein were identified. 【Result】 Fifteen protein spots were differentially expressed, and twelve proteins were identified after SEQUEST research and corresponding to ten proteins. Overall, these differential proteins were involved in molecular function contained binding, transport and catalytic activity; and participated in biological process contained cellular process, metabolic process, biological regulation and so on. 【Conclusion】 There was a significant difference at protein level in mammary gland from healthy dairy cows and clinical mastitic cows, differential proteins were related to infection of mastitis. It suggests that these proteins may be useful to explore the mammary gland involved in the pathophysiology, and to reveal the molecular mechanisms of clinical mastitis.