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美洲斑潜蝇不同寄主种群及地理种群间的β-tubulin基因序列分析
引用本文:杜予州,王莉萍,陆亚娟,郑福山,陆自强.美洲斑潜蝇不同寄主种群及地理种群间的β-tubulin基因序列分析[J].中国农业科学,2008,41(5):1542-1547.
作者姓名:杜予州  王莉萍  陆亚娟  郑福山  陆自强
作者单位:[1]扬州大学应用昆虫研究所,江苏扬州225009 [2]扬州进出境检验检疫局,江苏扬州225009
基金项目:国家自然科学基金 , 国家重点基础研究发展计划(973计划) , 江苏省科技攻关计划
摘    要: 【目的】通过对美洲斑潜蝇β-tubulin基因序列的比较,分析不同寄主种群和地理种群的遗传分化情况。【方法】对中国美洲斑潜蝇Liriomyza sativae Blanchard 5个寄主种群以及6个地理种群的β-微管蛋白基因(β-tubulin gene)进行测序,运用软件DNAStar和MEGA对美洲斑潜蝇不同地理种群及寄主种群的β-tubulin基因序列的遗传分歧及相似性进行分析,并建立系统发生树。【结果】DNAStar和MEGA两种软件对美洲斑潜蝇不同寄主种群以及地理种群间的遗传关系的分析可得到相似的结果。在美洲斑潜蝇寄主种群和地理种群序列变异分析中,共发现8个变异位点,序列长度比较保守,均没有碱基的插入或缺失。美洲斑潜蝇不同寄主种群之间以及地理种群之间的β-tubulin基因序列相似性极高,都在98%以上。【结论】虽然基于β-tubulin基因序列所显示的美洲斑潜蝇各寄主种群之间以及地理种群之间的遗传分化很小,但是其分化趋势分别与对寄主的嗜好程度以及地理分布基本吻合。

关 键 词:美洲斑潜蝇  寄主种群  地理种群  β-tubulin基因  遗传分化
收稿时间:2006-12-11
修稿时间:2006年12月11

Differentiation of Different Host-Populations and Geo-Populations of Liriomyza sativae Blanchard Based on β-tubulin Gene Sequences
DU Yu-zhou,WANG Li-ping,LU Ya-juan,ZHENG Fu-shan,LU Zi-qiang.Differentiation of Different Host-Populations and Geo-Populations of Liriomyza sativae Blanchard Based on β-tubulin Gene Sequences[J].Scientia Agricultura Sinica,2008,41(5):1542-1547.
Authors:DU Yu-zhou  WANG Li-ping  LU Ya-juan  ZHENG Fu-shan  LU Zi-qiang
Abstract:【Objective】To analysis the genetic differentiation of the host- and geo-populations of Liriomyza sativae Blanchard.【Method】Theβ-tubulin gene of 5 host-populations and 6 geo-populations of Liriomyza sativae Blanchard were sequenced. The identity and variation of β-tubulin gene sequenced were analyzed by biosoftware DNAStar and MEGA and the phylogenetic trees were constructed. 【Result】The results obtained by the biosoftware DNAStar and MEGA respectively were similar. The differences of β-tubulin gene among the host- and geo-populations of L.sativae were very low, with 8 variable sites and no insertions and deletions and the identities were all above 98%.【Conclusion】The trends of differentiation of host- and geo-populations of L. sativae were consistent with the hobby to hosts and the geographical distribution, respectively.
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