甘蓝型油菜DH群体苗期耐湿性的评价

 【目的】评价甘蓝型油菜双单倍体（doubled haploid,DH）群体苗期耐湿性,筛选极端耐湿性DH系。【方法】采用盆栽试验,以株高（PH）、根长（RL）、地上部干重(SDW)、根干重(RDW)、根冠比(R/S)、总干重（TDW）等6个性状的耐湿系数作为耐湿性评价指标,对甘蓝型油菜DH群体的118个株系及其亲本进行苗期耐湿性评价。【结果】（1）与对照相比,湿害严重抑制了甘蓝型油菜苗期的生长,6个评价指标均表现出显著差异,其中根干重的变异系数最大;（2）在对照和湿害条件下,群体各株系各性状均表现出超亲连续分离,大部分呈正态分布,湿害条件下的分离更为明显;（3）相关性分析表明,地上部干重、根干重和总干重耐湿系数可作为甘蓝型油菜苗期耐湿性的主要评价指标。【结论】本研究综合应用地上部干重耐湿系数、地上部干重耐湿系数和总干重耐湿系数3个评价指标,筛选出005、007、040为候选的极端耐湿性基因型,086、110、119等为候选的极端不耐湿基因型,为耐湿相关研究及育种提供新材料。

Evaluation of Waterlogging Tolerance in Rapeseed (Brassica napus L) DH Lines at Seedling Stage

Objective] The objective of the study was to evaluate waterlogging tolerance of the rapeseed (Brassica napus L.) doubled haploid (DH) population and to screen the most waterlogging-tolerant and waterlogging-sensitive DH lines. Method] For phenotyping, the rapeseed seedlings were grown in plastic pots. Six traits (plant height, root length, shoot dry weight, root dry weight, root/shoot ratio and total dry weight) together with WTC (waterlogging tolerance coefficient) of all the traits were investigated and analyzed. The waterlogging tolerance of the 118 DH lines and their parents were evaluated. Result] The results showed that: the six responsive traits changed significantly under the waterlogging conditions compared with the control in the experiment and the effects of waterlogging were much more severe on RDW than other traits. All of the six traits and WTC of all the traits showed transgressive and continuous distribution under both waterlogging and control conditions. The correlation among all the traits investigated in the study indicated that WTC of SDW, RDW and TDW could be used as available evaluation indices for waterlogging tolerance. Conclusion] Lines 005, 007, and 040 screened from the population are the most waterlogging-tolerant lines and 086, 110, and 119 are the most waterlogging-sensitive lines.