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| 四川省葛藤属根瘤菌的遗传多样性研究 | |
| 引用本文： | 陈强,陈文新,张小平,李登煜,K.Lindstrom.四川省葛藤属根瘤菌的遗传多样性研究[J].中国农业科学,2004,37(11):1641-1646. |
| 作者姓名： | 陈强 陈文新 张小平 李登煜 K.Lindstrom |
| 作者单位： | 1. 农业部农业微生物资源及其应用重点开放实验室,中国农业大学生物学院,北京100094;四川农业大学资源环境学院,雅安,625000 2. 农业部农业微生物资源及其应用重点开放实验室,中国农业大学生物学院,北京100094 3. 四川农业大学资源环境学院,雅安,625000 4. Department of Applied Chemistry and Microbiology,University of Helsinki,Finland Fin-00014 |
| 基金项目： | 国家重点基础研究发展计划资助项目(2001CB108905)和国家自然科学基金资助项目(3027001) |
| 摘 要： | 用AFLP、REP-PCR、23S rDNA PCR-RFLP等方法对分离自四川的23株葛藤属的根瘤菌的遗传特性进行了研究。结果表明，供试的葛藤根瘤菌遗传特性存在明显差异。 AFLP分析结果显示，23个菌株分为7个AFLP遗传群；在BOXAIR-PCR分析中，23株根瘤菌则被分为5个遗传群；对23S rDNA PCR-RFLP分析表明，葛藤根瘤菌分属于中慢生根瘤菌属（Mesorhizobium）、慢生根瘤菌属（Bradyrhizobium）2个属，聚类分析结果将其划分为5个遗传群。分离自四川省的葛藤根瘤菌存在较大的遗传多样性。 |
| 关 键 词： | 根瘤菌 遗传多样性 葛藤 AFLP |
| 收稿时间： | 2004-2-27 |
Genetic Diversity of Rhizobia Isolated from Pueraria spp.in Sichuan,China |
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| Abstract: | Twenty-three rhizobial strains isolated from Pueraria spp. in different sites of Sichuan province were studied by using AFLP, BOXAIR-PCR and 23S rDNA PCR-RFLP techniques. The results showed that genetic diversity among these rhizobia was existed. The analysis of AFLP revealed that, there were seven AFLP genotypes among these rhizobial strains; and BOXAIR-PCR suggested that, there were five genotypes existed. The results from 23S rDNA PCR-RFLP analysis revealed that, from the phylogeny point of view, these rhizobia belong to Mesorhizobium sp. and Bradyrhizobium sp., and however, most of them were different from the type strains of the related genera, which indicated that maybe there are putative new species existed. |
| Keywords: | Rhizobia Genetic diversity Pueraria spp AFLP |
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