福建省青枯雷尔氏菌脂肪酸多态性研究

 【目的】利用气相色谱技术检测福建省的40株青枯雷尔氏菌(Ralstonia solanacearum)菌株细胞内的脂肪酸，分析其脂肪酸分布的多态性；研究青枯雷尔氏菌脂肪酸多态性与青枯雷尔氏菌现有种下分化方法之间的关系。【方法】对40株青枯雷尔氏菌脂肪酸进行气相色谱分析，比较同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌脂肪酸的分布；对40株青枯雷尔氏菌脂肪酸进行聚类分析，分析聚成的各类青枯雷尔氏菌脂肪酸的特点以及脂肪酸多态性与其生理小种、生化型和致病性之间的关系。【结果】同一寄主分离的青枯雷尔氏菌和不同寄主分离的青枯雷尔氏菌，其脂肪酸都存在着明显的多态性；对40株青枯雷尔氏菌的脂肪酸进行聚类分析，可以聚成3类，即group Ⅰ、group Ⅱ和group Ⅲ；青枯雷尔氏菌生理小种1存在着不同的脂肪酸类群，青枯雷尔氏菌脂肪酸多态性与其生化型之间不存在相关性，但是脂肪酸和致病性之间存在一定的相关性：group Ⅰ为无致病性菌株，group Ⅱ为过渡性菌株，group Ⅲ为强致病性菌株。【结论】福建省青枯雷尔氏菌脂肪酸分布存在着明显的多态性；青枯雷尔氏菌脂肪酸多态性与致病性之间存在一定的相关性，脂肪酸有望成为青枯雷尔氏菌小种鉴定的新指标。

Polymorphism of Fatty Acid of Ralstonia solanacearum in Fujian Province

【Objective】Fatty acids of different strains of Ralstonia solanacearum (Rs) were detected by using gas chromatography and the polymorphism was analyzed in order to investigate the relationship between the polymorphism of fatty acid and taxonomies of Rs in existence.【Method】Fatty acids of 40 strains of Rs were detected by using gas chromatography. Different Rs strains isolated from the same host plant or from the different host plants were compared with their fatty acid distributions. The fatty acids from 40 strains of Rs were analyzed by cluster analysis and find out the characteristics of fatty acids about these groups, the relationships between the polymorphism of fatty acid and Rs race, biovar type and virulent type were separately investigated. 【Result】The result showed that the polymorphism of fatty acid of strains isolated from the same host plant or from the different host plants was evident based on fatty acid distribution, those 40 strains of Rs could be separated into three groups: group I, group II and group III. No relationship was found between the polymorphism of fatty acid and Rs race or Rs biovar type. But, there was a certain correlation between it and virulent type: group I was avirulent, group III was virulent and the other type was transition-virulent. 【Conclusion】The polymorphism of fatty acid of Rs was evident and there was a certain correlation between it and virulent type. Fatty acid might become a new index for taxonomy of Rs.