| 番茄根特异表达基因LeGRP2启动子的克隆及其在拟南芥的表达分析 |
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| 引用本文： | 李志邈,杨悦俭,杨飞,叶青静,王荣青,阮美颖,周国治,姚祝平,Yong-Ling Ruan.番茄根特异表达基因LeGRP2启动子的克隆及其在拟南芥的表达分析[J].中国农业科学,2010,43(9):1877-1882. |
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| 作者姓名： | 李志邈 杨悦俭 杨飞 叶青静 王荣青 阮美颖 周国治 姚祝平 Yong-Ling Ruan |
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| 作者单位： | （浙江省农业科学院蔬菜研究所/中澳作物改良研究中心）; |
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| 摘 要： | 目的克隆获得番茄根特异表达启动子,为利用基因工程技术创制番茄新种质奠定基础。方法利用Clontech公司的基因组步移(genome walking)技术,扩增番茄根特异表达基因LeGRP2的上游调控序列,并构建植物表达载体,利用农杆菌介导法转化拟南芥,以GUS为报告基因研究该调控序列的组织表达特异性。结果以番茄基因组DNA为模板,经过2次基因组步移,获得了LeGRP2基因上游1959bp的调控序列(GenBank登录号:EU262719),分析发现含有9个与根特异表达相关的顺式作用元件ROOTMOTIFTAPOX1。转基因拟南芥的组织化学染色分析表明,GUS基因主要在拟南芥的根部特异表达。结论克隆获得了番茄LeGRP2基因启动子,该启动子主要在转基因拟南芥根部表达GUS基因,具有较强的根表达特异性。
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| 关 键 词： | 番茄 根特异启动子 基因组步移 组织表达特异性 |
| 收稿时间： | 2009-08-13; |
Cloning of LeGRP2 Promoter from Tomato that Shows Root-Specific Expression in Arabidopsis |
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| LI Zhi-miao,YANG Yue-jian,YANG Fei,YE Qingqing,WANG Rong-qing,RUAN Mei-ying,ZHOU Guo-zhi,YAO Zhu-ping,Yong-Ling Ruan.Cloning of LeGRP2 Promoter from Tomato that Shows Root-Specific Expression in Arabidopsis[J].Scientia Agricultura Sinica,2010,43(9):1877-1882. |
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| Authors: | LI Zhi-miao YANG Yue-jian YANG Fei YE Qingqing WANG Rong-qing RUAN Mei-ying ZHOU Guo-zhi YAO Zhu-ping Yong-Ling Ruan |
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| Institution: | LI Zhi-miao1,YANG Yue-jian1,YANG Fei1,2,YE Qing-jing1,WANG Rong-qing1,RUAN Mei-ying1,ZHOU Guo-zhi1,YAO Zhu-ping1,Yong-Ling Ruan3 (1Institute of Vegetables,Zhejiang Academy of Agricultural Sciences/China-Australia Research Center for Crop Improvement,Hangzhou 310021,China,2College of Chemistry , Life Science,Zhejiang Normal University,Jinhua 321004,3School of Environmental , Life Sciences,University of Newcastle,Callaghan,NSW 2308,Australia) |
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| Abstract: | Objective The objective of the study was to clone a root-specific promoter from tomato for future use in generating new tomato materials through genetic engineering. Method Genomic walking technique was used to amplify the upstream regulatory sequence of LeGRP2 (glycine-rich protein), a tomato root-specific expression gene. To investigate the tissue expression pattern of the cloned regulatory sequence, an expression vector containing this sequence fused with GUS was constructed for transformation into Arabi... |
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| Keywords: | tomato (Solanum lycopersicum) root-specific promoter genomic walking tissue-specific expression |
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