低磷营养胁迫对小麦-长穗偃麦草附加系酸性磷酸酶同工酶的影响

 以中国春-长穗偃麦草二体异附加系为材料，用等电聚焦（IEF）方法研究低磷营养胁迫下叶片与根系组织内酸性磷酸酶（AcPh）同工酶的变化。结果表明，与正常生长条件（CK）下相比较，低磷营养胁迫导致AcPh同工酶的显著变化。叶片组织中，低磷胁迫诱导出的至少1条AcPh同工酶带主要在低Ph范围内,高pH范围内的AcPh同工酶带则与CK无明显差异。根系组织中则与此相反，高pH范围内诱导出至少3条新酶带，而低pH范围内，则主要是酶带的加强。此外，对低磷胁迫下叶片与根系组织内AcPh同工酶变化的生理意义进行了讨论。

Influence of Phosphorus Deficiency Stress on Acid Phosphatase Isozymes in Addition Lines Between Common Wheat and Agropyron elongatum

Using a set of alien disomic addition lines of Chinese Spring Agropyron elongatum (2n=2x=14,EE) as materials,the influence of phosphorus deficiency stress on acid phosphatase (AcPh) isozymes was studied by IEF analysis. The results indicated that AcPh isozymes significantly changed (the bands or new induced bands) in both leaf and root tissues under phosphorus deficiency stress in comparison with that under normal growth condition (CK). In leaf tissue,at least 1 band induced by the stress appeared within low pH ranges. In contrast to that,no significant differences appeared in AcPh isozymes under both CK and the stress conditions within high pH ranges. However,at least 3 induced bands were found within the range of high pH,and only the enhancement of the bands was performed within the low pH ranges in root tissue.In addition,the physiological significance of these changes of AcPh isozymes under the stress was discussed.