油菜田日本看麦娘对高效氟吡甲禾灵抗药性的研究

 【目的】明确油菜田日本看麦娘（Alopecurus japonicus Steud.）对高效氟吡甲禾灵（haloxyfop-R- methyl）的抗药性及对其它药剂的交互抗性水平。【方法】利用种子生物测定法和整株测定法测定了江苏、安徽11个点油菜田日本看麦娘对高效氟吡甲禾灵的抗药性，并用同样方法进行了相关药剂的交互抗性测定。【结果】两种方法测定结果基本相符。种子生物测定结果显示，江苏句容点日本看麦娘对此药剂的相对抗性倍数达61.13，整株测定结果为1 331.68，说明句容点的日本看麦娘对此药剂已产生明显抗性。交互抗性测定结果显示，日本看麦娘对芳氧基苯氧基丙酸酯类（aryloxyphenoxypropionates，简称AOPP）其它药剂已产生了不同程度的抗性，抗性水平依次为精吡氟禾草灵＞精喹禾灵＞精噁唑禾草灵；对具相同作用靶标的环已烯酮类（cyclohexanediones，简称CHD）除草剂烯禾啶的抗性水平相对较低。室内测定结果与田间实际反映情况相吻合。【结论】种子生物测定法可作为杂草抗性水平测定的方法之一。油菜田日本看麦娘对AOPP类高效氟吡甲禾灵已产生不同程度的抗药性，其中句容点抗性最高，对同类产品精吡氟禾草灵的交互抗性水平也最高；对与AOPP类除草剂具有不同结构、相同作用机制的CHD类烯禾啶交互抗性水平较低。

Study on Resistance of Alopecurus japonicus Steud. Populations to Haloxyfop-R-methyl in Oilseed Rape Fields

【Objective】 The objective of this study was to investigate the resistance level of Alopecurus japonicus Steud. to haloxyfop-R-methyl, which is an ACCase inhibitor belonging to aryloxyphenoxypropionates (AOPPs), and its cross-resistance level to other herbicides with the same target site. A. japonicus seeds were collected from different oilseed rape fields where haloxyfop-R-methyl had been continuously applied for different years. 【Method】 The resistance level of A. japonicus to haloxyfop-R-methyl were evaluated by seed-bioassay and whole-plant test. 【Result】 The results showed that similar results were obtained by both of methods. In these two methods, the resistance factors of A. japonicus to haloxyfop-R-methyl, which were collected from Jurong in Jiangsu province, were 61.13 and 1331.68 respectively, which demonstrated the emergence of A. japonicus population resistant to haloxyfop-R-methyl in fields. The results of cross-resistance revealed that this resistant population of A. japonicus was also resistant to other AOPPs, in which the resistance level was: fluazifop-p-butyl＞quizalofop-p-ethyl＞fenoxaprop-p-ethyl. However, a relative low resistance level of this resistant population to sethoxydim was observed, which was a cyclohexanedione (CHD) herbicide sharing the same target with AOPPs. And the results obtained in laboratory were agreed with the real fact in fields.