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快速鉴定甘薯品种抗旱性的生理指标及方法的筛选
引用本文:张明生,谈锋,张启堂.快速鉴定甘薯品种抗旱性的生理指标及方法的筛选[J].中国农业科学,2001,34(3):260-265.
作者姓名:张明生  谈锋  张启堂
作者单位:1. 贵州大学农学院,
2. 西南师范大学生命科学学院,
基金项目:国家“九五”科技攻关项目(96-002-02-17)
摘    要: 用不同浓度的聚乙二醇(PEG)对甘薯进行根际水分胁迫处理,研究了叶片相对含水量(RWC)、丙二醛(MDA)含量、超氧物歧化酶(SOD)活性及游离脯氨酸(Pro)含量的变化与品种抗旱性的关系。结果表明,25%PEG处理下,叶片RWC与品种抗旱性呈极显著正相关(r=0.783,P<0.01),MDA含量与品种抗旱性呈极显著负相关r=-0.848,P<0.01),SOD活性与品种抗旱性呈极显著正相关(r=0.777,P<0.01),Pro含量与品种抗旱性的关系不大。品种抗旱性愈强,叶片 RWC下降幅度及MDA含量上升幅度愈小,SOD活性增加幅度愈大。因此,通过测定 25%PEG处理下甘薯幼苗叶片的这些生理指标可实现品种抗旱性的室内快速鉴定。

关 键 词:甘薯  抗旱性  水分胁迫  生理指标  抗旱系数
文章编号:0578-1752(2001)03-0260-06
修稿时间:2000年7月24日

Physiological Indices for Rapid Identification of Sweet Potato Drought Resistance and Selection of Methods
ZHANG Ming-sheng,TAN Feng,ZHANG Qi-tang.Physiological Indices for Rapid Identification of Sweet Potato Drought Resistance and Selection of Methods[J].Scientia Agricultura Sinica,2001,34(3):260-265.
Authors:ZHANG Ming-sheng  TAN Feng  ZHANG Qi-tang
Abstract:The relationships between the changes of relative water content (RWC), malondialdehyde (MDA) contents, superoxide dismutase (SOD) activity and free proline contents in sweet potato leaves, and drought resistant ability under different concentration of polyethylene glycol (PEG) treatment applied at rhizosphere of sweet potato seedlings were studied. A highly positive correlation between RWC and drought resistance (r = 0. 783, P < 0. 01), a highly negative correlation between MDA contents and drought resistance (r= 0. 848, P < 0. 01), a highly positive correlation between SOD activity and drought resistance (r=0. 777, P<0. 01) was observed. Free proline contents in leaves did not relate obviously to the sweet potato drought resistance under 25 % PEG treatment. The stronger the drought resistance of variety, the less decrease of RWC and increase of MDA contents, the more increase of SOD activity. By determining these physiological indices in sweet potato leaves under 25% PEG treatment, rapid identification of drought resistant ability of different varieties can be obtained in the lab.
Keywords:Sweet potato  Drought resistance  Water stress  Physiological indices  Drought coefficient
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