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玉米小斑病菌T小种培养滤液对玉米抗病性的诱导
引用本文:马春红,翟彩霞,王立安,陈霞,郭秀林,崔四平,李广敏.玉米小斑病菌T小种培养滤液对玉米抗病性的诱导[J].中国农业科学,2005,38(8):1578-1584.
作者姓名:马春红  翟彩霞  王立安  陈霞  郭秀林  崔四平  李广敏
作者单位:1. 河北省农林科学院遗传生理研究所,石家庄,050051
2. 河北省农林科学院遗传生理研究所,石家庄,050051;河北师范大学生命科学学院,石家庄,050016
3. 河北师范大学生命科学学院,石家庄,050016
4. 河北省农林科学院,石家庄,050051
5. 河北省农林科学院遗传生理研究所,石家庄,050051;河北省农林科学院,石家庄,050051
基金项目:河北省自然科学基金项目(300163)和河北省农林科学院发展研究项目(A03-2-01-14)资助
摘    要: 以1对同核异质的玉米C103为试材,进行了利用低浓度玉米小斑病菌T小种培养滤液作为激发子来提高玉米抗病性的研究。结果表明, 经预处理叶片的病斑面积为(0.3±0.05)~(0.9±0.5)mm2,而对照为(23.1±8.7)mm2,差异极显著。同时还测定了与提高抗病性有关酶的变化,经0~96 h的动态检测,以1∶50的处理效果为最好,苯丙氨酸解氨酶(PAL)活性比对照平均提高了64.2%,过氧化物酶(POD)活性比对照平均提高了41.2%,有害物质丙二醛(MDA)的含量比对照平均降低了29.7%。从而说明低浓度培养滤液本身能够成功地作为激发子来诱导玉米的系统获得性抗性。

关 键 词:玉米小斑病菌T小种  粗毒素(培养滤液)  苯丙氨酸解氨酶  过氧化物酶  丙二醛  诱导抗性
收稿时间:2004-06-25
修稿时间:2004-06-25

Induced Resistance by the Filtration of Bipolaris maydis Race T Toxin Cultivation
MA Chun-hong,ZHAI Cai-xia,WANG Li-an,CHEN Xia,GUO Xiu-lin,CUI Si-ping,LI Guang-min.Induced Resistance by the Filtration of Bipolaris maydis Race T Toxin Cultivation[J].Scientia Agricultura Sinica,2005,38(8):1578-1584.
Authors:MA Chun-hong  ZHAI Cai-xia  WANG Li-an  CHEN Xia  GUO Xiu-lin  CUI Si-ping  LI Guang-min
Institution:MA Chun-hong1,ZHAI Cai-xia1,2,WANG Li-an2,CHEN Xia3,GUO Xiu-lin1,CUI Si-ping1,LI Guang-min1,3
Abstract:Resistance to maize southern leaf spot disease was induced by the low concentration of filtration of Bipolaris maydis race T cultivation in an experiment. The nuclear neterogeny corn C103 was used as the test material .The lesion area on the leaves was significant difference by connalysis The lesion areas on pretreated leaves were (0.3±0.05)-(0.9±0.5) mm2, but that in the control were (23.1±8.7)mm2. At the same time, the changes of peroxidase, phenylalanine, ammonialyase, malondialdenvde activities were determined. During 0-96 h inspection, phenylalanine, ammonialyase activities increased by 64.2%, peroxidase activities increased by 41.2%, but the malondialdenvde content decreased by 29.7% compared with the control. It seems that the low concentration of filftration of B. maydis race T cultivation itself can be used as an elicitor to enhance the induced resistance.
Keywords:Bipolaris maydis race T  Filtration of cultivation  PAL  POD  MDA  Induced resistance
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