中国棉花主栽品种DNA指纹图谱构建及SSR标记遗传多样性分析

目的]采用SSR标记构建2008年中国3大棉区8个棉花主产省份32份棉花主栽品种的DNA指纹图谱并进行遗传多样性分析.方法]从214对候选引物中筛选出36对多态性高、稳定性好且在染色体上分布均匀的引物作为核心引物,构建棉花主栽品种DNA指纹图谱.结果]36对SSR引物在32份材料中共扩增出142种多态性基因型,每...

Construction of DNA Fingerprinting and Analysis of Genetic Diversity with SSR Markers for Cotton Major Cultivars in China

【Objective】The aim of this study was to construct a DNA fingerprinting database of 32 cotton major cultivars from three main cotton regions (including eight main cotton production provinces), and the genetic diversity was analyzed based on simple sequence repeats (SSR).【Method】Thirty six evenly distributed SSR primer pairs with high polymorphisms and good repeatability were successfully screened out from 214 candidates to construct the fingerprinting database.【Result】Among the 32 varieties, 36 primer pairs had 142 polymorphic genotypes, and 3.94 genotypes were detected by each SSR primer pair on an average with the range from 2 to11. Ten cultivars had specific genotypes by 9 primer pairs. Thirty two major cultivars could be identified by 5 primer combinations at least. The genetic diversity analyzed by the software of NTSYS-pc V 2.10 indicated that Yangtze River valley cotton region had the highest polymorphic level, followed by Xinjiang, and the lowest was the varieties from the Yellow River valley. The genetic basis of conventional cultivars was narrower than that of hybrids. 【Conclusion】 Core primer combination method is better than distinct band method for the construction of DNA fingerprinting of cotton major cultivars in China. The genetic relationship of the cotton cultivars derived from the same district is similar to a certain extent.