水溶性灵芝肽在动物体外的抗氧化活性

【目的】研究水溶性灵芝肽在动物体外的抗氧化活性。【方法】用丙二醛(MDA)试剂盒测定MDA含量，用分光光度法测定大鼠血清自氧化溶血及小鼠肝线粒体肿胀度。【结果】当水溶性灵芝肽的剂量为0.25 mg?ml-1时，对大鼠红细胞自氧化溶血的抑制率达62.33%，对溶血过程中MDA生成的抑制率达91.24%；在有或无自由基诱导剂（Fe2+或H2O2）时，0.35 mg?ml-1的水溶性灵芝肽均能极显著抑制小鼠肝匀浆中MDA的生成（P＜0.01），且抑制率大致相当，为60%左右；当水溶性灵芝肽剂量为1.00 mg?ml-1时，对线粒体肿胀度的抑制率为90.52%，对线粒体中MDA生成的抑制率达65.42%，且呈明显剂量-效应关系。【结论】水溶性灵芝肽在体外具有明显的抗氧化作用。

The Antioxidative Activity of Water-Soluble Peptides from Ganoderma lucidum in vitro Test

Abstract: The antioxidative activity of Ganoderma lucidum peptides (GLP) in vitro test was studied. The reagent kits were used to determine the content of malondialdehyde (MDA). The auto-hemolysis of mice red blood cells (RBC) and the swelling of mice liver mitochondria were observed by means of spectrophotometer. The results showed that the inhibiting rate of 0.25 mg·ml-1 GLP on the auto-hemolysis of mice RBC was 62.3% and the inhibiting rate on the MDA formation in mice RBC was 91.2%. 0.35 mg ml-1 GLP could extremely significantly prevent the MDA formation in mice liver homogenate ( p<0.01) with or without free radicals inducers (Fe2+or H2O2). They had the near inhibiting rate-around 60%. When 1.00 mg·ml-1 GLP was added, the inhibiting rate on the swelling of mice liver mitochondria was 90.5% and the inhibiting rate on the MDA formation in mice liver mitochondria was 65.4% in a dose-dependent manner. Conclusion: GLP was a good antioxidant in vitro test.