| 基因枪转化小麦悬浮细胞参数的优化 |
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| 引用本文： | 董云洲,贾士荣.基因枪转化小麦悬浮细胞参数的优化[J].中国农业科学,1998,31(6):79-81. |
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| 作者姓名： | 董云洲 贾士荣 |
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| 作者单位： | 中国农业科学院生物技术研究中心，北京 100081 |
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| 摘 要： | 小麦是我国的重要粮食作物，其遗传工程因受转基因技术的限制而进展缓慢。国际上，自１９９２年Ｖａｓｉｌ等〔７〕用基因枪技术首次得到转基因小麦以来，现在又有几例成功的报道〔３，４，８，９〕。国内成功的报道极少，有的即使得到了转基因植株，其频率也很低。导致小...
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| 关 键 词： | 小麦 悬浮细胞 基因枪 GUS基因短暂表达 |
| 修稿时间： | 1997-07-18 |
Optimization of Parameters Influencing Gene Delivery Into Cell Suspension Cultures of Wheat by Microprojectile Bombardment |
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| Dong Yunzhou,Jia Shirong.Optimization of Parameters Influencing Gene Delivery Into Cell Suspension Cultures of Wheat by Microprojectile Bombardment[J].Scientia Agricultura Sinica,1998,31(6):79-81. |
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| Authors: | Dong Yunzhou Jia Shirong |
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| Abstract: | An embryogenic suspension culture was established by culturing calli derived from immature embryos of wheat in M9 medium which was then used for optimization of different factors of particle bombardment affecting transient expression of GUS gene. The JQ 700 high velocity particle gun and the plasmid pBI121 were used in this study. The factors influencing the transient expression of GUS gene are optimized. |
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| Keywords: | Wheat Suspension cells Particle bombardment Transient expression of GUS gene |
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