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中国部分地区高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究
引用本文:张建武,庄金山,袁世山.中国部分地区高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究[J].中国农业科学,2008,41(6):1822-1831.
作者姓名:张建武  庄金山  袁世山
作者单位:中国农业科学院上海兽医研究所/中国动物卫生与流行病学中心上海分中心猪传染病防治研究室/农业部动物寄生虫学重点开放实验室,上海200232
基金项目:中央级院所社会公益性项目 , 上海科技兴农项目
摘    要: 【目的】对来自2006年猪病流行爆发地的组织样品进行多种病毒的检测,确定引起本次流行的致病病原及PRRSV的分子流行病学特征。【方法】利用(RT-)PCR对采集的样品进行PRRSV北美型和欧洲型、猪圆环病毒2型(PCV2)、猪血凝性脑脊髓炎病毒(PHEV)、日本乙型脑炎病毒(JEV)以及瘟病毒的检测,对PRRSV阳性的进行ORF5的序列测定并分析。【结果】检测到16份PRRSV北美型阳性样品,PCV2阳性样品2份,其它病毒均未检到。PRRSV ORF5序列分析表明笔者分离到的PRRSV株可分为2个群。【结论】(1)PRRSV与2006年肆虐中国养猪业的猪病流行有直接关系;(2)2006年PRRSV流行株主要是以JX0612株为代表,但不同遗传特征的PRRSV也在流行。

关 键 词:高致病性猪繁殖与呼吸综合征病毒  ORF5  分子流行病学调查  遗传进化分析
收稿时间:2007-5-21

Molecular Epidemiology Study on High Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Some Regions of China
ZHANG Jian-wu,ZHUANG Jin-shan,YUAN Shi-shan.Molecular Epidemiology Study on High Pathogenic Porcine Reproductive and Respiratory Syndrome Virus in Some Regions of China[J].Scientia Agricultura Sinica,2008,41(6):1822-1831.
Authors:ZHANG Jian-wu  ZHUANG Jin-shan  YUAN Shi-shan
Abstract:【Objiective】Various viruses were detected from tissue samples of porcine high fever syndrome. Etiology of porcine high fever syndrome and molecule epidemiology of porcine reproductive and respiratory syndrome virus were determined. 【Method】PRRSV North America type and Europe type, porcine circovirusⅡ, porcine haemagglutinating encephalomyelitis virus, Japanese encephalitis virus and Pestivirus were identified by (RT-)PCR from tissue samples. Sequence of PRRSV ORF5 was amplified and analyzed. 【Result】16 PRRSV North America type positive samples and PCV2 positive samples were detected; other viruses were not detected. Sequence analysis of PRRSV ORF5 indicated: PRRSV isolated strains could be divided into two groups. 【Conclusion】1)PRRSV was the causative agent of porcine high fever syndrome in China. 2) PRRSV prevalence strain was strain represented by JX0612, however defferent genetic characteristics PRRSV strains also spread in China.
Keywords:Porcine high fever syndromeHigh pathogenicity porcine reproductive and respiratory syndrome virus  ORF5  Molecule epidemiologyGenetic evolution analysis
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