水分胁迫下离体烟叶中脂氧合酶活性、水杨酸与茉莉酸积累的关系

 采用河南农业大学设计的电热式温湿度自控烤烟箱 ,研究水分胁迫下离体烟叶中脂氧合酶活性、水杨酸(SA)与茉莉酸 (JA)积累的关系。结果表明 ,水分胁迫下茉莉酸积累的同时 ,脂氧合酶 (LOX)活性也上升 ,两者呈极显著正相关 ;去加愈创木酸 (NDGA)是LOX专一抑制剂 ,在抑制LOX活性的同时也阻断了水分胁迫下茉莉酸的积累 ;无细胞体系加入LOX可直接引起茉莉酸的积累。这表明LOX很可能是JA生物合成及胁迫诱导JA积累的一种关键酶 ,水杨酸抑制茉莉酸积累。

Relationship Between LOX Activity and SA and JA Accumulations in Tobacco Leave Under Water Stress

The relationship between LOX(lipoxygenase)activity and SA(salicylic acid) and JA(jasmonic acid) accumulation in tobacco leaves was studied. The results showed that LOX activity and JA content increased simultaneously along with lost of water; NDGA, a specific inhibitor of LOX, inhibited simultaneously both the activity of LOX and the enhancement of JA level under water stress. Likewise adding LOX to tobacco cell free system led to the increase of JA content. The result suggested that LOX may be a key enzyme in JA biosynthesis under water stress. SA inhibited the enhancement of JA level under water stress.