| 姬松茸~(60)Co辐射菌株J_3若干特性研究 |
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| 引用本文： | 翁伯琦,江枝和,黄挺俊,陈坚,郑伟文.姬松茸~(60)Co辐射菌株J_3若干特性研究[J].中国农业科学,2003,36(9):1065-1070. |
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| 作者姓名： | 翁伯琦 江枝和 黄挺俊 陈坚 郑伟文 |
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| 作者单位： | 福建省农业科学院,福州,350003 |
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| 基金项目： | 福建省科技厅资助项目(2002N047,2003N044) |
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| 摘 要： | 采用60 Co辐射诱变技术 ,获得姬松茸新菌株J3 。连续栽培其产量比原菌株高 70 %以上。经氨基酸、脂肪酸组分分析表明 ,J3 菌株子实体的营养价值优于原菌株 ;经扫描电镜观察发现J3 菌株菌丝体有明显竹节状结构 ,RAPD分析显示其PCR指纹图谱有异于原菌株。由此判断 ,J3 菌株是一个富有开发前景的突变株。
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| 关 键 词： | 姬松茸 60Co辐射 超微结构 RAPD分析 营养评价 |
| 修稿时间： | 2003年1月2日 |
Study on Several Characteristics of Strain J3 Agaricus blazei Murril Irradiated by 60Co |
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| WENG Bo-qi,JIANG Zhi-he,HUANG Ting-jun,CHEN Jian,ZHENG Wei-wen.Study on Several Characteristics of Strain J3 Agaricus blazei Murril Irradiated by 60Co[J].Scientia Agricultura Sinica,2003,36(9):1065-1070. |
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| Authors: | WENG Bo-qi JIANG Zhi-he HUANG Ting-jun CHEN Jian ZHENG Wei-wen |
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| Abstract: | The new strain J 3 of Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations all increased more than 70% than its original strain.The components analysis of amino acid and fatty acid showed that the nutrient evaluation of strain J 3 were higher than its original strain. The apparent nodules of strain J 3 hyphae were observed through scanning electron microscope. The results of RAPD analysis indicated that there was a great difference of PCR fingerprints between J 3 and its original strain. It is concluded that J 3 is a mutant. |
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| Keywords: | Agaricus blazei Murril 60Co irradiation Ultrastructure RAPD analysis Nutrient evaluation |
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