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山羊高繁殖力候选基因BMP15的RFLP分析
引用本文:焦彩兰,储明星,王金玉,刘忠慧,方丽,叶素成.山羊高繁殖力候选基因BMP15的RFLP分析[J].扬州大学学报(农业与生命科学版),2006,27(3):31-34.
作者姓名:焦彩兰  储明星  王金玉  刘忠慧  方丽  叶素成
作者单位:1. 扬州大学,动物科学与技术学院,江苏,扬州,225009
2. 中国农业科学院,北京畜牧兽医研究所,北京,100094
基金项目:国家自然科学基金资助项目(30540052),北京市自然科学基金资助项目(6062023)
摘    要:以控制R om ney Inverdale绵羊和R om ney H anna绵羊高繁殖力的骨形态发生蛋白15(BM P 15)基因为候选基因,采用PCR-RFLP方法检测BM P 15基因在高繁殖力山羊品种(济宁青山羊)以及低繁殖力山羊品种(内蒙古绒山羊、安哥拉山羊、波尔山羊)中的多态性,同时研究该基因对济宁青山羊高繁殖力的影响。结果表明:BM P 15基因在济宁青山羊、内蒙古绒山羊、安哥拉山羊和波尔山羊中既未发生与Inverdale绵羊相同的V 31D突变,也未发生与H anna绵羊相同的Q 23T er突变。这表明BM P 15基因这2个突变位点对济宁青山羊的高繁殖力没有显著影响。

关 键 词:山羊  高繁殖力  骨形态发生蛋白15基因  PCR-RFLP
文章编号:1671-4652(2006)03-0031-04
收稿时间:2005-06-20
修稿时间:2005年6月20日

RFLP analysis on bone morphogenetic protein 15 as a candidate gene for prolificacy in goats
JIAO Cai-lan,CHU Ming-xing,WANG Jin-yu,LIU Zhong-hui,FANG Li,YE Su-cheng.RFLP analysis on bone morphogenetic protein 15 as a candidate gene for prolificacy in goats[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2006,27(3):31-34.
Authors:JIAO Cai-lan  CHU Ming-xing  WANG Jin-yu  LIU Zhong-hui  FANG Li  YE Su-cheng
Abstract:Bone morphogenetic protein 15(BMP15) gene which controls the fecundity of Inverdale ewes and Hanna ewes was studied as a candidate gene for prolificacy of Jining grey goats.Polymorphism of BMP15 gene was detected in both high fecundity goat breed(Jining grey goats) and low fecundity goat breeds(Inner Mongolia cashmere goats,Angora goats and Boer goats) by PCR-RFLP.The result showed that both V31D mutation in Inverdale ewes and Q23Ter mutation in Hanna ewes of BMP15 gene did not exist in four goat breeds detected.This result indicated these two mutations of BMP15 gene had no significant effect on prolificacy of Jining grey goats.
Keywords:goat  prolificacy  bone morphogenetic protein 15 gene  PCR-RFLP
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