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禽源性大肠杆菌HPI irp2基因序列的扩增及比较
引用本文:金文杰,郑志明,王倩倩,秦爱建,刘岳龙,邵红霞.禽源性大肠杆菌HPI irp2基因序列的扩增及比较[J].扬州大学学报(农业与生命科学版),2005,26(3):5-7.
作者姓名:金文杰  郑志明  王倩倩  秦爱建  刘岳龙  邵红霞
作者单位:扬州大学江苏省动物预防医学重点实验室,江苏扬州225009
基金项目:国家高技术研究发展计划项目(8632003AA22141)
摘    要:从养殖场发病家禽中分离到多株禽源性大肠杆菌(Escherichia coli),用其中YZG040515、NTLFC040402、CHZ031016菌株提取细菌高致病性染色体DNA,根据耶尔森菌高致病性毒力岛(HPI)irp2基因设计引物,用PCR扩增出278 bp基因片段。将PCR产物克隆到pGEM-T-easy中,经EcoR I酶切鉴定为阳性者进行序列测定。结果表明:该基因片段与GeneBank中公布的耶尔森菌HPI irp2基因的同源性高达98%以上。证明禽源性大肠杆菌中具有耶尔森菌毒力岛HPI基因序列,结合临床发病情况和流行病学,提示该毒力岛与日趋严重的家禽大肠杆菌病可能有一定的相关性。

关 键 词:禽源性大肠杆菌  耶尔森菌  高致病性毒力岛  克隆比较
文章编号:1671-4652(2005)03-0005-03
修稿时间:2005年6月16日

Comparison of partial sequence of avian E.coli strains with Yersinia HPI
JIN Wen-jie,ZHENG Zhi-ming,WANG Qian-qian,QIN Ai-jian,LIU Yue-long,SHAO Hong-xia.Comparison of partial sequence of avian E.coli strains with Yersinia HPI[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2005,26(3):5-7.
Authors:JIN Wen-jie  ZHENG Zhi-ming  WANG Qian-qian  QIN Ai-jian  LIU Yue-long  SHAO Hong-xia
Abstract:Avian Escherichia coli strains were isolated from chicken with colibacillosis.The chromosome DNAs of E.coli were extracted.A cDNA fragment was amplified by PCR with primers designed according to the sequence of the Yersinia high-pathogenicity island(HPI) irp2 gene published in Genbank.All of 3 PCR products were cloned into pGEM-T-easy and sequenced.The results showed that the sequences from 3 E.coli strains shared more than 98% homology with the irp2 gene of Yersinia HPI.It indicates that the HPI has existed in avian E.coli strains and it may be related to the pathogenicity of poultry E.coli in poultry.
Keywords:avian  Escherichia coli  Yersinia  high-pathogenicity island(HPI)  sequence analysis
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