| 鸡传染性支气管炎病毒单克隆抗体的研制与鉴定 |
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| 引用本文： | 万洪全,许益民,王永坤,朱国强,孙龙生,栾世璐,严维巍.鸡传染性支气管炎病毒单克隆抗体的研制与鉴定[J].扬州大学学报(农业与生命科学版),1997(2). |
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| 作者姓名： | 万洪全 许益民 王永坤 朱国强 孙龙生 栾世璐 严维巍 |
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| 作者单位： | 江苏农学院动物医学系 |
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| 基金项目： | 江苏省“九五”农业科技攻关项目 |
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| 摘 要： | 用经蔗糖密度梯度离心纯化的鸡传染性支气管炎病毒（ＩＢＶ，Ｍ４１）作为抗原免疫ＢＡＬＢ／ｃ小鼠，通过常规淋巴细胞杂交瘤技术产生杂交瘤细胞，经ＥＬＩＳＡ筛选，获得了４株能分泌特异性抗ＩＢＶ单克隆抗体的细胞系，分别命名为４ＡＣ１、４ＡＦ１、６ＤＨ８及２ＤＨ１０。４株单抗的亚类都属于ＩｇＧ２ｂ，在Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ试验中，４ＡＦ１、６ＤＨ８２株单抗特异性地识别ＩＢＶ的核衣壳蛋白（Ｎ）；经ＥＬＩＳＡ测定，各株单抗４０ｈ培养上清效价达１０－２～１０－４，第７天腹水效价达１０－５～１０－６，４ＡＦ１、６ＤＨ８与所试７个ＩＢＶ标准株及２个ＩＢＶ分离株都发生反应
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| 关 键 词： | 鸡 传染性 支气管炎 病毒 抗体／单克隆 |
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST AVIAN INFECTIOUS BRONCHITIS VIRUS |
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| Wan Hongquan Xu Yiming Wang Yongkun,Zhu Guoqiang et al.PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST AVIAN INFECTIOUS BRONCHITIS VIRUS[J].Journal of Yangzhou University:Agricultural and Life Science Edition,1997(2). |
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| Authors: | Wan Hongquan Xu Yiming Wang Yongkun Zhu Guoqiang |
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| Abstract: | Four hybridoma cell lines, designated as 4AC 1, 4AF 1, 6DH 8, 2DH 10 , respectively, which can stably secrete monoclonal antibodies against infectious bronchitis virus(IBV), were established through common hybridoma technique. All these McAbs belong to IgG2b isotype. Two of them, 4AF 1 and 6DH 8, defined an approximately 54kd protein band in Western blotting analysis, indicating that they are nucleocapsid protein specific McAbs. Both 4AF 1 and 6DH 8 strongly reacted with the 9 tested IBV strains (7 reference strains, 2 isolated strains) in ELISA. This confirms that 4AF 1 and 6DH 8 will serve as valuable tools in detection of IBV. |
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| Keywords: | domestic fowl infectious bronchitis virus antibody/monoclonal |
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