| 脑部特异表达rtTA调控元件转基因小鼠的构建 |
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| 引用本文： | 章蓉,孙强,徐桂芳,董娟,金宇娟,江楠.脑部特异表达rtTA调控元件转基因小鼠的构建[J].扬州大学学报(农业与生命科学版),2008,29(1):16-19. |
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| 作者姓名： | 章蓉 孙强 徐桂芳 董娟 金宇娟 江楠 |
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| 作者单位： | 华东师范大学,脑功能基因组学研究所,上海,200062 |
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| 摘 要： | 为获得一种可调控基因表达系统,避免传统转基因在胚胎发育早期表达的毒性作用或致死作用以及基因过度表达而造成难于分析的复杂多表型,应用分子生物学方法将四环素反式激活子rtTA克隆到仅在脑部能被激活的钙调蛋白依赖性蛋白激酶CaM kinase Ⅱα亚基启动子之后,将其通过显微注射方法注入FVB小鼠受精卵雄原核中获得四环素调控系统中反应组分的转基因工具鼠.经PCR及Southern检测,最终得到3只阳性小鼠(1公2母),表达检测结果显示,其中仅2只(1公1母)后代检测到rtTA表达产物.结果表明已成功构建四环素调控系统中的转基因工具鼠,为今后研究其他脑部基因功能提供便利.
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| 关 键 词： | 小鼠 转基因 rtTA CaM kinaseⅡ |
Construction of forebrain-specific rtTA expression transgenic mouse |
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| ZHANG Rong,SUN Qiang,XU Gui-fang,DONG Juan,JIN Yu-juan,JIANG Nan.Construction of forebrain-specific rtTA expression transgenic mouse[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2008,29(1):16-19. |
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| Authors: | ZHANG Rong SUN Qiang XU Gui-fang DONG Juan JIN Yu-juan JIANG Nan |
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| Institution: | Shanghai Inst of Brain Fun Gen;East of China Univ;Shanghai 200062;China |
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| Abstract: | Conditional gene expression may help to avoid not only blocking analysis of phenotype caused by toxic or lethal effects in embryonic development early time,but also complicating phenotype caused by ubiquitous overexpression,so we utilized the benefits of an optimized tet-on system to construct p279-rtTA with molecular biology method,then it was transferred into FVB mice by pronucleus microinjection to produce transgenic mouse,to this constructed forebrain-specific rtTA transgenic tool mouse.Through PCR and ... |
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