| 诱导性免疫共刺激分子转基因小鼠模型的建立 |
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| 引用本文： | 孙强,岳挺,冯洁,董娟,邓忠彬,李厚达.诱导性免疫共刺激分子转基因小鼠模型的建立[J].扬州大学学报(农业与生命科学版),2005,26(1):13-15. |
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| 作者姓名： | 孙强 岳挺 冯洁 董娟 邓忠彬 李厚达 |
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| 作者单位： | 扬州大学,比较医学中心,江苏,扬州,225009;苏州大学,医学生物技术研究所,江苏,苏州,215007 |
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| 基金项目： | 国家"十五"科技攻关项目(2001BA70113) |
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| 摘 要： | 利用RT—PCR方法从人扁桃体的激活T细胞mRNA中扩增出诱导性免疫共刺激分子(ICOS)cDNA,继而将ICOS基因插入到pEGFP—N3中得到含ICOS和GFP融合蛋白的pEGFP—ICOS。将外源基因pEGF、P—ICOS注射至FVB小鼠原核中,其胚胎移植到同期发情的假孕受体产出后代,经PCR和Southern检测获得阳性转基因小鼠。试验共注射移植胚胎117枚,出生小鼠43只,检出阳性小鼠2只,该转基因已稳定遗传至F3代,说明建立了ICOS转基因小鼠模型。
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| 关 键 词： | 诱导性免疫共刺激分子 转基因 动物模型 |
| 文章编号： | 1671-4652(2005)01-0013-03 |
| 修稿时间： | 2004年9月23日 |
The establishment of ICOS transgenic mice |
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| SUN Qiang,YUE Ting,FENG Jie,DONG Juan,DENG Zhong-bin,LI Hou-da.The establishment of ICOS transgenic mice[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2005,26(1):13-15. |
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| Authors: | SUN Qiang YUE Ting FENG Jie DONG Juan DENG Zhong-bin LI Hou-da |
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| Abstract: | Human icos(Inducible costimulator)cDNA was cloned from activated T cells of human tonsil by RT-PCR, then it was inserted into pEGFP-N3 vector and got pEGFP-ICOS which contained a fuse protein ICOS-GFP. The pEGFP-ICOS gene was microinjected to the pronuclear of FVB mouse embryos, then transfered the embryos to the foster mother. Positive founders was confirmed by PCR and Southern. 117 injected embryos were transferred and got 43 offsprings, two of them were positive. Now the transgenic mouse was maintaimed in F3, and it was an ICOS transgenic mouse model. |
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| Keywords: | ICOS transeenic animal model |
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