| 5个绵羊群体微卫星多态性分析 |
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| 引用本文： | 柴文琼,成述儒,靳建华,罗玉柱.5个绵羊群体微卫星多态性分析[J].扬州大学学报(农业与生命科学版),2012,33(1):38-42. |
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| 作者姓名： | 柴文琼 成述儒 靳建华 罗玉柱 |
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| 作者单位： | 甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室/研究测试中心,甘肃兰州,730070 |
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| 基金项目： | 国家自然科学基金资助项目，国家“十一五”科技支撑计划项目，国家农业科技成果转化项目，甘肃省农业科技创新项目，甘肃省农业生物技术研究与应用开发项目，甘肃省自然科学基金资助项目，甘肃农业大学科技创新基金资助项目 |
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| 摘 要： | 采用微卫星DNA标记对甘肃高山细毛羊、滩羊等5个绵羊群体(125只个体)的5个微卫星座位等位基因进行检测,分析5个绵羊群体的遗传多样性和亲缘关系。结果表明:5个绵羊群体中共发现78个等位基因,其中在甘肃高山细毛羊肃南县群体中发现的等位基因数最多(57个),滩羊最少(43个)。多态信息含量、期望杂合度和观察杂合度的数据分析表明:在研究的5个绵羊群体中甘肃高山细毛羊天祝县群体和肃南县群体的遗传多样性较丰富,而滩羊的遗传多样性相对较低。DA遗传距离和DS遗传距离构建的邻接(NJ)系统发育树均表明:甘肃高山细毛羊天祝县群体和肃南县群体与藏羊的亲缘关系较近,为一类;而滩羊与小尾寒羊的遗传距离较近,为另一类。
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| 关 键 词： | 绵羊 微卫星DNA 遗传多样性 系统发育 |
Analysis of microsatellite polymorphism in five sheep populations |
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| CHAI Wen-qiong
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CHEN Shu-ru
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JIN Jian-hua
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LUO Yu-zhu.Analysis of microsatellite polymorphism in five sheep populations[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2012,33(1):38-42. |
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| Authors: | CHAI Wen-qiong CHEN Shu-ru JIN Jian-hua LUO Yu-zhu |
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| Institution: | (Gansu Key Lab of Herb Anim Biotech / Faculty of Anim Sci-Tech / Res and Test Cen,Gansu Agric Univ Lanzhou,730070,China) |
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| Abstract: | In order to evaluate the genetic diversity and relationship of Sannan Gansu Alpine Merino,Tianzhu Gansu Alpine Merino,Tan sheep,Small Tailed Han sheep and Tibetan sheep,five microsatellite DNA marks were used in five sheep populations(125 individuals).The results showed that the number of alleles detected in Tianzhu Gansu Alpine Merino are most,was 57,and the least in Tan sheep,was only 43.The populations PIC and heterozygosity analysis showed that Tianzhu Gansu Alpine Merino and Sunan Gansu Alpine Merino had the richest genetic diversity,but Tan sheep genetic diversity was lower than others.The results of genetic distance and phylogenetic tree analysis showed that the relationship of Sannan Gansu Alpine Merino,Tianzhu Gansu Alpine Merino and Tibetan sheep was nearer.Moreover,the relationship of Small Tailed Han sheep and Tan sheep was nearer. |
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| Keywords: | sheep microsatellite DNA genetic diversity phylogenetics |
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