秋稻品种Aus373广亲和基因的RFLP分析 |
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引用本文: | 陆驹飞,严长杰,朱立煌,顾铭洪.秋稻品种Aus373广亲和基因的RFLP分析[J].扬州大学学报(农业与生命科学版),1997(4). |
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作者姓名: | 陆驹飞 严长杰 朱立煌 顾铭洪 |
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作者单位: | 江苏农学院农学系,中国科学院遗传研究所 |
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基金项目: | 国家高新技术发展研究计划资助 |
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摘 要: | 利用Aus373/IR36//Balila和Aus373/IR36//秋光2个三交F1群体对秋稻品种Aus373的广亲和性进行了遗传分析,发现这两个群体内不同个体的小穗育性有着明显的分离,呈单峰连续分布。小穗育性的高低与稃尖色之间存在连锁关系。用Aus373/IR36//Balila群体构建第6染色体的RFLP框架图,并对亲和性基因进行了定位。在第6染色体上发现有2个来自Aus373区段对小穗育性有直接贡献,其联合效应可以解释总变异的38.4%。其中位于G200和C基因之间的可能就是Sn5,位于G122和G329之间的可能是另一个亲和性基因。
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关 键 词: | 水稻 基因+定位/RFLP分析 广亲和性 |
RFLP ANALYSIS ON WIDE COMPATIBILITY GENES IN AUS373 OF ECOTYPE AUS OF RICE |
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Abstract: | Genetic analysis on the wide compatibility in Aus373 of Ecotype Aus of Rice was conducted based on triple crosses of Aus373 /IR36//Balilla and Aus373/IR36// Akihikari The distribution of spikelet fertility in the two populations was continous unimodal The spikelet fertility was correlated with apiculus color An RFLP linkage map of chromosome 6 including 13 loci was constructed with triple cross of Aus373/IR36//Balilla Two loci associated with wide compatibility on chromosome 6 were identified by interval mapping approach The one was located between chromogene(C) and molecular marker G200 and the other between G122 and G329, which were responsible for 19 89% and 13 64% of phynotypic variation respectively and for 38 4% combinatively The results indicate that there are two wide compatibility genes on chromosome 6 in Aus373 The one between C and G200 is S n 5, the other is temporarily designated as S n x |
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Keywords: | paddy gene positioning/RFLP analysis wide compatibility |
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