| 鹅GH基因内含子3多态性及其与体重和屠宰性能相关分析 |
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| 引用本文： | 陈清,赵文明,程金花,乔娜,王学斌,徐琪,陈国宏.鹅GH基因内含子3多态性及其与体重和屠宰性能相关分析[J].扬州大学学报(农业与生命科学版),2008,29(1):41-44. |
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| 作者姓名： | 陈清 赵文明 程金花 乔娜 王学斌 徐琪 陈国宏 |
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| 作者单位： | 扬州大学,动物科学与技术学院,江苏,扬州,225009 |
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| 基金项目： | 国家科技支撑计划重点项目
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江苏省高校自然科学基础研究面上项目 |
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| 摘 要： | 根据鸭GH基因序列设计引物扩增籽鹅、皖西白鹅和狮头鹅3个地方鹅品种GH基因内含子3片段,并运用PCR-SSCP技术进行单核苷酸多态性分析.结果表明: 鹅GH基因内含子3存在多态性,得到AA、AB和BB 3种基因型.3个品种中等位基因B占绝对优势,且主要以BB型存在.品种内基因型分布差异不显著,3个品种均处于Hardy-Weinberg平衡状态(P>0.05);而品种间,籽鹅与皖西白鹅基因型分布差异显著.基因型与4～10周龄体重、屠体重、半净膛重等19个性状呈显著相关.这19个性状的BB型个体均显著或极显著高于AA型个体,除胸肌率外,BB型个体各性状均值最高,AB型次之,AA型最小.故初步推断BB基因型可作为地方鹅品种高生产性能的分子标记.
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| 关 键 词： | 鹅 GH基因 PCR-SSCP 体重 屠体性状 |
| 文章编号： | 1671-4652(2008)01-0041-04 |
| 修稿时间： | 2007年12月11 |
Polymorphism of intron 3 of growth hormone gene and its relationship with bodyweight and carcass traits in goose |
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| CHEN Qing,ZHAO Wen-ming,CHENG Jin-hua,QIAO Na,WANG Xue-bin,XU Qi,CHEN Guo-hong.Polymorphism of intron 3 of growth hormone gene and its relationship with bodyweight and carcass traits in goose[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2008,29(1):41-44. |
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| Authors: | CHEN Qing ZHAO Wen-ming CHENG Jin-hua QIAO Na WANG Xue-bin XU Qi CHEN Guo-hong |
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| Institution: | Coll of Anim Sci and Tech;Yangzhou Univ;Yangzhou 225009;China |
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| Abstract: | This study investigated single nucleotide polymorphism(SNP)of growth hormone(GH)gene in three local goose breeds(Zi goose,Wanxi white goose,Shitou goose),the primers for intron 3 in GH gene were designed according to the homologous sequence of duck and the SNPs were detected by PCR-SSCP method.Three genotypes(AA,AB and BB)within all breeds were found.The B allele was more frequent in three goose breeds and mainly existed in the form of BB genotype.Within breeds the frequencies of GH gene fited with Hardy-We... |
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| Keywords: | goose GH gene PCR-SSCP bodyweight carcass trait |
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