| 哈蟆油薄层色谱指纹图谱研究 |
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| 引用本文： | 王永生,赵晓欧,白雪洁,张小兰,杨晓虹.哈蟆油薄层色谱指纹图谱研究[J].特产研究,2009,31(3):31-33,36. |
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| 作者姓名： | 王永生 赵晓欧 白雪洁 张小兰 杨晓虹 |
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| 作者单位： | [1]吉林大学,吉林长春130021 [2]长春中医药大学,吉林长春130021 |
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| 基金项目： | 国家中医药管理局科研基金项目 |
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| 摘 要： | 目的研究哈蟆油药材的薄层色谱（TLC）指纹图谱,为科学评价及有效控制其质量提供可靠方法。方法样品经预处理后点于硅胶G预制板上,以正己烷∶丙酮∶冰醋酸（8∶2∶1）为展开剂,磷钼酸试液为显色剂,于625nm处进行吸收扫描。结果11种不同来源的哈蟆油样品薄层色谱指纹图谱所形成的全谱图像显示,不同品种有很高的相似性。结论TLC指纹图谱是控制哈蟆油质量的良好方法。
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| 关 键 词： | 哈蟆油 TLC 指纹图谱 |
TLC Fingerprinting Analysis of Oviductus Ranae |
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| Institution: | Wang Yong-sheng, Zhao Xiao-ou, Bai Xue-jie, Zhang Xiao-lan, Yang Xiao-hong ( 1.Jilin University Jilin, Changchun, 130021 China; 2.Changchun University of TCM, Changchun, 130021 China) |
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| Abstract: | Objective To set up a sensitive and specific TLC method for controlling the quality of Oviductus Ranae.Method The sample solution was applied to the commercial precoated silica gel plates, solvent system included n-Hexane-acetone-glacial acetic acid（8：2：1）, phophosphoric acid test solution was as visualizing reagent, and quantitative analysis was carried out using UV absorptive scanning at 625nm.Result Consistent among eleven batches of Oviductus Ranae was demonstrated by TLC fingerprint image and the profiles generated by digital scanning.Conclusion It is a good method quality of Oviductus Ranae that can be controlled effectively by TLC fingerprint. |
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| Keywords: | TLC |
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