气相色谱-质谱法测定十味香鹿胶囊挥发油的化学成分

采用气相色谱-质谱联用法对十味香鹿胶囊的挥发油成分进行分析。采用2010年版《中国药典》挥发油测定法对十味香鹿胶囊挥发油进行提取,以毛细管柱进行分析,面积归一化法测定百分含量,并对其化学成分进行鉴定。色谱柱为Agilent19091M-436型弹性石英毛细管色谱柱(0.25mm×30m,0.25μm);程序升温:60℃,以10℃/min升至280℃,保持12min。进样口温度为280℃。质谱条件:EI离子源,电子能量70eV,离子源温度230℃;扫描范围20~500amu。共检出33个化合物,鉴定出其中的22个化学成分,相对含量超过5%的成分有棕榈酸(54.107%)、顺-9-十八烯酸甲酯(8.092%)、反-9-十八碳烯酸(7.285%)、(Z,Z)-9,12-十八碳二烯酸(6.026%)、9-反十六碳烯酸甲酯(5.456%),同时鉴定出具有镇痛作用的丁香酚甲醚(0.172%)。十味香鹿胶囊中挥发油成分主要包含棕榈酸、烯酸以及烯酸甲酯类成分等。

GC-MS Analysis of Volatile oil Constituents from Shiwei Xianglu Capsule

To analyze the volatile oil components from Shiwei Xianglu Capsule by GC-MS.The volatile oil components were extracted and isolated with steam distillation method according to ChP(2010).The chemical compositions were separated by GC capillary column chromatography,and the relative contents were analyzed by GC-MS.The GC-MS analysis with an Agilent 19091M-436 capillary column(30m×0.25mm,0.25μm);The inlet temperature was maintained at 280℃.The column oven was begun at 60℃,then programmed from 60 to 280℃ at 10℃/min,held for 12 min.Mass spectrometer conditions were:ionization mode,EI;electron energy 70eV;Ion source temperature 230℃.The mass selective detector was operated in the TIC mode,mass scan range,20～500 amu.33 compounds were detected and 22 compounds of them were identified.And the compounds that the relative contents were more than 5% were Methyl hexadec-9-enoate(5.456%),palmitic acid(54.107%),(Z)-9-Octadecenoic acid methyl ester(8.092%),(Z,Z)-9.12-Octadecadienoic acid(6.026%),(E)-9-Octadecenoic acid(7.285%).The main components in the oil were palmitic acid,olefine acid,ene acid methyl ester.