梅花鹿生茸骨膜干细胞可溶性总蛋白抗体的制备及滴度测定

为了获取梅花鹿生茸骨膜干细胞可溶性总蛋白抗体。本试验培养了梅花鹿生茸骨膜干细胞,从该细胞中提取可溶性总蛋白。以其可溶性总蛋白为抗原,通过淋巴结和皮下注射两种方式免疫试验动物,注射的抗原量分别为70μg/只和280μg/只。分别于末次免疫后第10天和第20天采血,分离血清,通过ELISA试验检测血清中抗体滴度。结果表明,280μg皮下免疫和70μg淋巴结免疫均获得了较好的免疫效果,末次免疫后第10天的抗体滴度较高。

Preparation and Detection of Total Soluble Protein Antibody for Antlerogenic Periosteum Stem Cells in Sika Deer(Cervus nippon Temminck)

Sika deer （Cervus nippon Temminck ）antlerogenic periosteal （AP）cells were cultured in order to produce polyclonal antibody. Total soluble protein of AP cells was extracted as antigen. New Zealand white rabbits were inoculated through lymph node injection or sub- cutaneous injection. The dosage of injection was 70 μ g and 280 μ g respectively. The blood was collected 10 days and 20 days after the last inoculation respectively, and the serum thereof was isolated. The antibody liter of each serum was detected by ELISA. The results showed that 70 μ g antigen lymph node injection and 280 μ g antigen subcutaneous injection were resuhed good immune-reaction ,antibody titer of the serum collected 10 days after the last inoculation was higher than that of 20 days.