东北原生种猕猴桃种质RAPD研究

对东北原生种猕猴桃的软枣、狗枣及葛枣猕猴桃RAPD研究结果表明:3个种DNA多态性较强。聚类分析表明:不同种间遗传距离较远,软枣与狗枣猕猴桃间亲缘相对较近,此二者与葛枣猕猴桃相对较远;种内雄株与雌株之间遗传距离相对较远,这种结果可能与花型有关;软枣猕猴桃种内和种间杂交品系分为3组,第1组为软枣雌株,第2组为软枣杂交品系(软枣雌株×美味猕猴桃),第3组为软枣雄株。3种在起源上接近,狗枣与葛枣的带型比软枣更为丰富,推测此2种可能较软枣更为进化。本研究建立的指纹图谱可用于东北原生种猕猴桃的种质鉴定和亲缘关系分析。

A Study of RAPD on Germplasm of Northeastern Actinidia chinensis in China

The results showed a very high polymorphism of DNA among the three species of A.arguta,A.kolomikta and A.pilygama of the study of RAPD on Northeastern Actinidia chinensis in China.The clustering analyses showed that the genetic distance of different species was comparatively far, the genetic distance of A.arguta and A.kolomikta was near while the genetic distance of A.polygama was not so near as that of A.arguta and A.kolomikta;the genetic distance of male plants(A.arguta and A.kolomikta) was comparatively far with female plant of A.arguta which result maybe was the difference of flower type;there existed three groups in A.arguta,the first group was female plant,the second group was crossing systems and the third group was male plant.The three species were near in origin,the DNA polymorphism of A.kolomikta and A.pilygama were abundant than that of A.arguta and we inferred that those two species were evolutionary types of A.arguta.The finger map of Actinidia chinensis made in this study can be used to germ plasm identification and relationship analyses.