利用冷冻切片法与石蜡切片法对水貂组织切片进行优化研究

应用冷冻切片法和石蜡包埋切片法，对水貂体组织进行切片后HE染色观察；并在冷冻切片方法中应用3种不同的固定剂，对固定剂的同定效果进行对比分析。结果表明，石蜡切片法更适合于脑组织，而冰冻切片法更适合于皮肤组织。冰冻切片方法中3种固定剂的固定效果比较，同定NA（无水乙醇80mL、甲醛10％10mL、冰醋酸10mL）优于固定剂C（10％的福尔马林溶液）与固定剂B（甲醛40％15mL、无水乙醇85mL）。

Optimization Analysis of Slicing Methods to Mink Tissues by Frozen and Paraffin Section

Using frozen and paraffin section methods to the body tissues of minks,then HE staining was used for observation;comparing analyzed the effects of three different fixative in the frozen sections.The results showed that: Paraffin method was more suitable for brain tissue,and frozen section method was more suitable for skin tissue;the effects of three difference fixatives to frozen section methods,fixative A(anhydrous alcohol 80 mL、Formaldehyde 10% 10 mL,glacial acetic acid 10 mL) was better than fixative C(formalin 10%) and fixative B(Formaldehyde 40%15 mL, anhydrous alcohol 85 mL).