| 新人参二醇血浆样品制备方法初探 |
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| 引用本文： | 赵俊艳,刘继华,朱枫,孟勤,尹建元,刘莹,倪加勉.新人参二醇血浆样品制备方法初探[J].特产研究,2010,32(2):46-48,61. |
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| 作者姓名： | 赵俊艳 刘继华 朱枫 孟勤 尹建元 刘莹 倪加勉 |
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| 作者单位： | 1. 吉林大学白求恩制药厂,吉林长春,130021
2. 吉林大学药学院,吉林长春,130021 |
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| 摘 要： | 建立新人参二醇血浆样品制备方法。以高效液相色谱法检测小鼠血浆提取物中新人参二醇的含量,确定小鼠血浆预处理方法。结果石油醚、乙醚和醋酸乙酯等萃取法制备的血浆样品中新人参二醇提取率低;甲醇萃取法、SPEC18固相萃取法制备的血浆样品提取率均较高,但甲醇萃取制备的样品中杂质峰干扰较大,而SPEC18固相萃取法制备的血浆样品中杂质峰对样品无干扰。SPEC18固相萃取法可作为新人参二醇血浆样品的制备方法。
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| 关 键 词： | 新人参二醇 血浆样品 溶剂萃取 固相萃取 |
Preliminary Studies on the Preparation Method of NPD plasma samples |
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| ZHAO Jun-yan,LIU Ji-hua,ZHU Feng,MENG Qin,YIN Jian-yuan,LIU Ying,NI jia-mian.Preliminary Studies on the Preparation Method of NPD plasma samples[J].Special Wild Economic Animal and Plant Research,2010,32(2):46-48,61. |
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| Authors: | ZHAO Jun-yan LIU Ji-hua ZHU Feng MENG Qin YIN Jian-yuan LIU Ying NI jia-mian |
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| Institution: | 1.Bai Qiu En Medicinal Production Factory of Jilin University,Changchun 130021,China;2.College of Pharmacy,Jilin University,Changchun 130021,China) |
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| Abstract: | Objective To establish the preparation method of extracting New Panaxadiol（NPD）from Rice plasma samples.Methods The content of NPD in the Rice plasma was determined by high-performance liquid chromatography,and the pre-process method of the Rice plasma was obtained.Results：The extraction rates by petroleum ether,ethyl ether and ethyl acetate were lower than by methanol or by solid-phase extraction method.The impurity had interference with NPD in methanol extracts,but impurity had no interference with NPD prepared by solid-phase method.Conclusion：Solid-phase method with SPEC18 is the most suitable for preparing the NPD plasma samples. |
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| Keywords: | New Panaxadiol Plasma samples Solvent extraction Solid-phase extraction |
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